PUBLICATION

Parametric functional maps of visual inputs to the tectum

Authors
Nikolaou, N., Lowe, A.S., Walker, A.S., Abbas, F., Hunter, P.R., Thompson, I.D., and Meyer, M.P.
ID
ZDB-PUB-121102-9
Date
2012
Source
Neuron   76(2): 317-324 (Journal)
Registered Authors
Abbas, Fatima, Hunter, Paul, Meyer, Martin, Nikolaou, Nikolas, Walker, Alison
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Axons/physiology
  • Brain Mapping*
  • Calcium/metabolism
  • Calmodulin/genetics
  • Green Fluorescent Proteins/genetics
  • Larva
  • Myosin-Light-Chain Kinase/genetics
  • Peptide Fragments/genetics
  • Retina/cytology
  • Retina/physiology
  • Retinal Ganglion Cells/physiology
  • Superior Colliculi/cytology
  • Superior Colliculi/physiology*
  • Visual Fields/physiology*
  • Visual Pathways/physiology*
  • Zebrafish
PubMed
23083735 Full text @ Neuron
Abstract

How features of the visual scene are encoded in the population activity of retinal ganglion cells (RGCs) targeting specific regions of the brain is not well understood. To address this, we have used a genetically encoded reporter of presynaptic function (SyGCaMP3) to record visually evoked activity in the population of RGC axons innervating the zebrafish tectum. Using unbiased voxel-wise analysis of SyGCaMP3 signals, we identify three subtypes of direction-selective and two subtypes of orientation-selective retinal input. Composite parametric functional maps generated across many larvae show laminar segregation of direction- and orientation-selective responses and unexpected retinotopic biases in the distribution of functional subtypes. These findings provide a systematic description of the form, organization, and dimensionality of visual inputs to the brain and will serve as a platform for understanding emergent properties in tectal circuits associated with visually driven behavior.

Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping