beta-catenin/Wnt signaling controls progenitor fate in the developing and regenerating zebrafish retina
- Authors
- Meyers, J.R., Hu, L., Moses, A., Kaboli, K., Papandrea, A., and Raymond, P.A.
- ID
- ZDB-PUB-120830-17
- Date
- 2012
- Source
- Neural Development 7(1): 30 (Journal)
- Registered Authors
- Meyers, Jason, Raymond, Pamela
- Keywords
- none
- MeSH Terms
-
- Retina/cytology*
- Retina/growth & development*
- Retina/injuries
- Retina/metabolism
- Wnt Signaling Pathway/drug effects
- Wnt Signaling Pathway/genetics
- Wnt Signaling Pathway/physiology*
- Stem Cells/drug effects
- Stem Cells/physiology*
- beta Catenin/genetics
- beta Catenin/metabolism*
- Embryo, Nonmammalian
- Enzyme Inhibitors/pharmacology
- Eye Proteins/metabolism
- Mutation/genetics
- Cell Cycle/drug effects
- Cell Cycle/genetics
- SOX Transcription Factors/genetics
- SOX Transcription Factors/metabolism
- Gene Expression Regulation, Developmental/drug effects
- Gene Expression Regulation, Developmental/genetics
- Benzazepines/pharmacology
- Animals, Genetically Modified
- Dose-Response Relationship, Drug
- Cell Differentiation/drug effects
- Cell Differentiation/genetics
- Retinal Rod Photoreceptor Cells
- Time Factors
- Neurogenesis/drug effects
- Neurogenesis/genetics
- Green Fluorescent Proteins/genetics
- Indoles/pharmacology
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism
- Larva
- Heterocyclic Compounds, 3-Ring/pharmacology
- Cell Proliferation/drug effects
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish
- Neuroglia/drug effects
- Neuroglia/physiology
- Nerve Regeneration/drug effects
- Nerve Regeneration/genetics
- Nerve Regeneration/physiology*
- Animals
- PubMed
- 22920725 Full text @ Neural Dev.
Background
The zebrafish retina maintains two populations of stem cells: first, the germinal zone or ciliary marginal zone (CMZ) contains multipotent retinal progenitors that add cells to the retinal periphery as the fish continue to grow; second, radial glia (Muller cells) occasionally divide asymmetrically to generate committed progenitors that differentiate into rod photoreceptors, which are added interstitially throughout the retina with growth. Retinal injury stimulates Muller glia to dedifferentiate, re-enter the cell cycle, and generate multipotent retinal progenitors similar to those in the CMZ to replace missing neurons. The specific signals that maintain these two distinct populations of endogenous retinal stem cells are not understood.
Results
We used genetic and pharmacological manipulation of the beta-catenin/Wnt signaling pathway to show that it is required to maintain proliferation in the CMZ and that hyperstimulation of beta-catenin/Wnt signaling inhibits normal retinal differentiation and expands the population of proliferative retinal progenitors. To test whether similar effects occur during regeneration, we developed a method for making rapid, selective photoreceptor ablations in larval zebrafish with intense light. We found that dephosphorylated beta-catenin accumulates in Muller glia as they re-enter the cell cycle following injury, but not in Muller glia that remain quiescent. Activation of Wnt signaling is required for regenerative proliferation, and hyperstimulation results in loss of Muller glia from the INL as all proliferative cells move into the ONL.
Conclusions
beta-catenin/Wnt signaling is thus required for the maintenance of retinal progenitors during both initial development and lesion-induced regeneration, and is sufficient to prevent differentiation of those progenitors and maintain them in a proliferative state. This suggests that the beta-catenin/Wnt cascade is part of the shared molecular circuitry that maintains retinal stem cells for both homeostatic growth and epimorphic regeneration.