Expression of complement component C7 and involvement in innate immune responses to bacteria in grass carp
- Authors
- Shen, Y., Zhang, J., Xu, X., Fu, J., and Li, J.
- ID
- ZDB-PUB-120529-32
- Date
- 2012
- Source
- Fish & shellfish immunology 33(2): 448-454 (Journal)
- Registered Authors
- Keywords
- complement component C7, Aeromonas hydrophila, expression, immune
- MeSH Terms
-
- Aeromonas hydrophila/immunology
- Amino Acid Sequence
- Animals
- Base Sequence
- Carps/classification
- Carps/genetics*
- Carps/immunology*
- Complement C7*/genetics
- Complement C7*/immunology
- Fish Diseases/immunology*
- Gene Expression Profiling
- Gene Expression Regulation
- Gram-Negative Bacterial Infections/immunology
- Gram-Negative Bacterial Infections/veterinary*
- Immunity, Innate*
- Molecular Sequence Data
- Phylogeny
- Promoter Regions, Genetic/genetics
- Sequence Alignment
- PubMed
- 22617254 Full text @ Fish Shellfish Immunol.
Activation of the complement system, which forms a major part of the innate immune system, results in the formation of the terminal complement complex. The complement component, C7, plays an integral role in the assembly of this complex within target cell membranes. In this study, C7 was isolated and characterized from grass carp, an important cultured fish in China. The predicted amino acid sequence of C7 cDNA (2650 bp) exhibited 55.4 and 48.3% homology with trout C7-1 and zebrafish C7, respectively. The grass carp C7 gene was consisted of 18 exons and 17 introns. C7 gene expression was detected in the trunk kidney, liver, head kidney, skin, spleen, heart and intestine. Significant changes in C7 transcript expression (>20-fold) were detected following Aeromonas hydrophila infection, indicating C7 involvement in innate immune responses to bacteria in teleost fish.