PUBLICATION
Methodology for reconstructing early zebrafish development from in-vivo multiphoton microscopy
- Authors
- Luengo-Oroz, M., Rubio-Guivernau, J., Faure, E., Savy, T., Duloquin, L., Olivier, N., Pastor, D., Ledesma-Carbayo, M., Debarre, D., Bourgine, P., Beaurepaire, E., Peyrieras, N., and Santos, A.
- ID
- ZDB-PUB-120105-19
- Date
- 2012
- Source
- IEEE transactions on image processing : a publication of the IEEE Signal Processing Society 21(4): 2335-2340 (Journal)
- Registered Authors
- Duloquin, Louise, Savy, Thierry
- Keywords
- cell lineage tree, in-vivo 4D imaging, multiphoton microscopy, viscious watershed, zebrafish
- MeSH Terms
-
- Algorithms
- Animals
- Cell Tracking/methods*
- Image Enhancement/methods
- Image Interpretation, Computer-Assisted/methods*
- Imaging, Three-Dimensional/methods*
- Microscopy, Fluorescence, Multiphoton/methods*
- Pattern Recognition, Automated/methods*
- Reproducibility of Results
- Sensitivity and Specificity
- Zebrafish/anatomy & histology*
- Zebrafish/embryology*
- PubMed
- 22155959 Full text @ IEEE Trans. Image Process.
Citation
Luengo-Oroz, M., Rubio-Guivernau, J., Faure, E., Savy, T., Duloquin, L., Olivier, N., Pastor, D., Ledesma-Carbayo, M., Debarre, D., Bourgine, P., Beaurepaire, E., Peyrieras, N., and Santos, A. (2012) Methodology for reconstructing early zebrafish development from in-vivo multiphoton microscopy. IEEE transactions on image processing : a publication of the IEEE Signal Processing Society. 21(4):2335-2340.
Abstract
Investigating cell dynamics during early zebrafish embryogenesis requires specific image acquisition and analysis strategies. Multiharmonic microscopy -second (SHG) and third harmonic generation (THG)- allows imaging cell divisions and cell membranes in unstained zebrafish embryos from 1-cell to 1K-cell stage. This article presents the design and implementation of a dedicated image processing pipeline (tracking and segmentation) for the reconstruction of cell dynamics during these developmental stages. This methodology allows the reconstruction of the cell lineage tree including division timings, spatial coordinates, and cell shape until the 1K-cell stage with minute temporal accuracy and m spatial resolution. Data analysis of the digital embryos provides an extensive quantitative description of early zebrafish embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping