PUBLICATION
Sox9 Is Upstream of MicroRNA-140 in Cartilage
- Authors
- Nakamura, Y., He, X., Kato, H., Wakitani, S., Kobayashi, T., Watanabe, S., Iida, A., Tahara, H., Warman, M.L., Watanapokasin, R., and Postlethwait, J.H.
- ID
- ZDB-PUB-111117-17
- Date
- 2012
- Source
- Applied Biochemistry and Biotechnology 166(1): 64-71 (Journal)
- Registered Authors
- He, Xinjun, Postlethwait, John H., Watanabe, Sumiko
- Keywords
- microRNA-140 (miR-140), Sox9, cartilage, zebrafish, T/C-28, siRNA, in situ hybridization, RT-PCR
- MeSH Terms
-
- Animals
- Binding Sites
- Cartilage/growth & development*
- Cartilage/metabolism
- Chondrocytes/metabolism
- Chondrogenesis/genetics
- Embryonic Development/genetics
- Gene Expression Regulation, Developmental
- Humans
- In Situ Hybridization
- MicroRNAs/genetics*
- MicroRNAs/metabolism*
- Phylogeny
- Protein Binding
- RNA, Small Interfering/genetics
- SOX9 Transcription Factor/genetics*
- SOX9 Transcription Factor/metabolism*
- Ubiquitin-Protein Ligases/genetics
- Zebrafish/genetics
- Zebrafish/growth & development*
- PubMed
- 22052544 Full text @ Appl. Biochem. Biotechnol.
Citation
Nakamura, Y., He, X., Kato, H., Wakitani, S., Kobayashi, T., Watanabe, S., Iida, A., Tahara, H., Warman, M.L., Watanapokasin, R., and Postlethwait, J.H. (2012) Sox9 Is Upstream of MicroRNA-140 in Cartilage. Applied Biochemistry and Biotechnology. 166(1):64-71.
Abstract
MicroRNA-140 (miR-140) is specifically expressed in developing cartilage tissues. We have previously reported that miR-140 plays an important role during palatal cartilage development by modulating platelet-derived growth factor receptor alpha (pdgfra) in zebrafish. However, the regulatory mechanism of miR-140 in cartilage is still unknown. Using developing zebrafish, sox9a mutant (sox9a-/-) and sox9b mutant (sox9b-/-) zebrafish and SOX9 small interfering RNA in human chondrocytes, T/C-28 cells, we found that miR-140 is regulated by the cartilage master transcription regulator Sox9 in zebrafish and mammalian cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping