PUBLICATION
Viral Insertion Mutants
- Authors
- Burgess, S., and Lin, S.
- ID
- ZDB-PUB-110915-1
- Date
- 2011
- Source
- ZFIN Direct Data Submission : (Unpublished)
- Registered Authors
- Burgess, Shawn, Lin, Shuo
- Keywords
- none
- MeSH Terms
- none
- PubMed
- none
Citation
Burgess, S., and Lin, S. (2011) Viral Insertion Mutants. ZFIN Direct Data Submission. . (http://zfin.org).
Abstract
Protocol Summary:
Moloney Murine Leukemia Virus (MLV) core particles were produced in a GT186 packaging cell line (1) (Genbank accession: JN244738.1) and pseudotyped with Vesicular Stomatitis Virus (VSV) envelope (G) protein. The virus was super-concentrated by high-speed centrifugation and resuspended in PBS. Virus was injected into T/AB-5 embryos at the 1-4000 cell stage (approximately 3hpf). Embryos were heat shocked at 37°C for 1.5 hours and then raised by normal animal husbandry to sexual maturity. Mature injected adults were either inbred or outcrossed to generate heterozygous transgenic male fish. The F1 males were raised to sexual maturity and then the sperm samples were cryopreserved; simultaneously genomic DNA was isolated from the tailfin and the genomic locations of the retroviral integrations was identified by a combination of linker-mediated PCR and Illumina paired-end sequencing.
Errata / Notes
1) J Virol. 2002 Mar;76(5):2192-8.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping