PUBLICATION
Differentiated skeletal cells contribute to blastema formation during zebrafish fin regeneration
- Authors
- Sousa, S., Afonso, N., Bensimon-Brito, A., Fonseca, M., Simões, M., Leon, J., Roehl, H., Cancela, M.L., and Jacinto, A.
- ID
- ZDB-PUB-110901-8
- Date
- 2011
- Source
- Development (Cambridge, England) 138(18): 3897-3905 (Journal)
- Registered Authors
- Roehl, Henry
- Keywords
- fin regeneration, skeletal regeneration, blastema formation, dedifferentiation, zebrafish
- MeSH Terms
-
- Cell Dedifferentiation/genetics
- Cell Dedifferentiation/physiology
- Bone and Bones/cytology
- Bone and Bones/metabolism
- Bone and Bones/physiology*
- Osteogenesis/genetics
- Cell Proliferation
- Gene Expression Profiling
- Regeneration/genetics
- Regeneration/physiology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Cell Differentiation/genetics
- Cell Differentiation/physiology
- Animals
- Gene Expression
- Bone Development/genetics
- Animal Fins/metabolism
- Animal Fins/physiology*
- Wound Healing/genetics
- Wound Healing/physiology
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish/physiology*
- Animals, Genetically Modified
- PubMed
- 21862555 Full text @ Development
Citation
Sousa, S., Afonso, N., Bensimon-Brito, A., Fonseca, M., Simões, M., Leon, J., Roehl, H., Cancela, M.L., and Jacinto, A. (2011) Differentiated skeletal cells contribute to blastema formation during zebrafish fin regeneration. Development (Cambridge, England). 138(18):3897-3905.
Abstract
The origin of cells that generate the blastema following appendage amputation has been a long-standing question in epimorphic regeneration studies. The blastema is thought to originate from either stem (or progenitor) cells or differentiated cells of various tissues that undergo dedifferentiation. Here, we investigate the origin of cells that contribute to the regeneration of zebrafish caudal fin skeletal elements. We provide evidence that the process of lepidotrichia (bony rays) regeneration is initiated as early as 24 hours post-amputation and that differentiated scleroblasts acquire a proliferative state, detach from the lepidotrichia surface, migrate distally, integrate into the blastema and dedifferentiate. These findings provide novel insights into the origin of cells in epimorphic appendage regeneration in zebrafish and suggest conservation of regeneration mechanisms between fish and amphibians.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping