PUBLICATION

Differentiated skeletal cells contribute to blastema formation during zebrafish fin regeneration

Authors
Sousa, S., Afonso, N., Bensimon-Brito, A., Fonseca, M., Simões, M., Leon, J., Roehl, H., Cancela, M.L., and Jacinto, A.
ID
ZDB-PUB-110901-8
Date
2011
Source
Development (Cambridge, England)   138(18): 3897-3905 (Journal)
Registered Authors
Roehl, Henry
Keywords
fin regeneration, skeletal regeneration, blastema formation, dedifferentiation, zebrafish
MeSH Terms
  • Animals
  • Cell Differentiation/genetics
  • Cell Differentiation/physiology
  • Animals, Genetically Modified
  • Animal Fins/metabolism
  • Animal Fins/physiology*
  • Gene Expression Profiling
  • Cell Dedifferentiation/genetics
  • Cell Dedifferentiation/physiology
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish/physiology*
  • Cell Proliferation
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Gene Expression
  • Regeneration/genetics
  • Regeneration/physiology*
  • Bone and Bones/cytology
  • Bone and Bones/metabolism
  • Bone and Bones/physiology*
  • Bone Development/genetics
  • Wound Healing/genetics
  • Wound Healing/physiology
  • Osteogenesis/genetics
PubMed
21862555 Full text @ Development
Abstract
The origin of cells that generate the blastema following appendage amputation has been a long-standing question in epimorphic regeneration studies. The blastema is thought to originate from either stem (or progenitor) cells or differentiated cells of various tissues that undergo dedifferentiation. Here, we investigate the origin of cells that contribute to the regeneration of zebrafish caudal fin skeletal elements. We provide evidence that the process of lepidotrichia (bony rays) regeneration is initiated as early as 24 hours post-amputation and that differentiated scleroblasts acquire a proliferative state, detach from the lepidotrichia surface, migrate distally, integrate into the blastema and dedifferentiate. These findings provide novel insights into the origin of cells in epimorphic appendage regeneration in zebrafish and suggest conservation of regeneration mechanisms between fish and amphibians.
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