PUBLICATION

GDNF family ligand RET receptor in the brain of adult zebrafish

Authors
Lucini, C., D'Angelo, L., Patruno, M., Mascarello, F., de Girolamo, P., and Castaldo, L.
ID
ZDB-PUB-110816-19
Date
2011
Source
Neuroscience letters   502(3): 214-8 (Journal)
Registered Authors
Keywords
RET, central nervous system, teleost fish
MeSH Terms
  • Aging/genetics
  • Aging/metabolism
  • Animals
  • Brain/cytology
  • Brain/metabolism*
  • Brain Chemistry/genetics
  • Glial Cell Line-Derived Neurotrophic Factor/metabolism*
  • Glial Cell Line-Derived Neurotrophic Factor/physiology
  • Isoenzymes/biosynthesis
  • Isoenzymes/genetics
  • Isoenzymes/metabolism
  • Ligands
  • Multigene Family*/genetics
  • Neurons/cytology
  • Neurons/enzymology
  • Neurons/metabolism
  • Proto-Oncogene Proteins c-ret/biosynthesis
  • Proto-Oncogene Proteins c-ret/genetics*
  • Proto-Oncogene Proteins c-ret/metabolism*
  • RNA Splicing/genetics
  • RNA, Messenger/metabolism
  • Species Specificity
  • Zebrafish
PubMed
21839141 Full text @ Neurosci. Lett.
Abstract
RET is a tyrosine kinase receptor, and transduces signaling by family of glial cell line-derived neurotrophic factor ligands (GFLs). RET is involved in the development of enteric nervous system, of sympathetic, parasympathetic, motor and sensory neurons. RET exists in two main isoforms originated by differential splicing, RET9 and RET51; phylogenetic studies have shown that the RET gene is conserved across vertebrates. The aim of this study was to investigate the RET expression within the brain of zebrafish, using immunohistochemistry, western blotting and RT-PCR. In homogenate brains both RET protein and mRNA were observed. RET immunoreactivity was widespread in neurons and neural processes of all the major regions of the brain. These results demonstrate the occurrence of RET and suggest an involvement of GDNF family ligands in the brain of adult zebrafish.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping