PUBLICATION
Identification of housekeeping genes suitable for gene expression analysis in the zebrafish
- Authors
- Casadei, R., Pelleri, M.C., Vitale, L., Facchin, F., Lenzi, L., Canaider, S., Strippoli, P., and Frabetti, F.
- ID
- ZDB-PUB-110207-14
- Date
- 2011
- Source
- Gene expression patterns : GEP 11(3-4): 271-6 (Journal)
- Registered Authors
- Casadei, Raffaella, Frabetti, Flavia
- Keywords
- Housekeeping gene, bactin2, Zebrafish, Quantitative relative RT-PCR
- MeSH Terms
-
- Animals
- Bone and Bones/metabolism
- Brain/metabolism
- Eye/metabolism
- Female
- Gene Expression Profiling*
- Muscles/metabolism
- Organ Specificity
- Ovary/metabolism
- RNA, Messenger/genetics*
- RNA, Messenger/metabolism
- RNA, Messenger/standards
- Reverse Transcriptase Polymerase Chain Reaction
- Skin/metabolism
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 21281742 Full text @ Gene Expr. Patterns
Citation
Casadei, R., Pelleri, M.C., Vitale, L., Facchin, F., Lenzi, L., Canaider, S., Strippoli, P., and Frabetti, F. (2011) Identification of housekeeping genes suitable for gene expression analysis in the zebrafish. Gene expression patterns : GEP. 11(3-4):271-6.
Abstract
Housekeeping (HK) genes are constitutively expressed in order to maintain cellular function. They produce the minimal essential transcripts necessary for normal cellular physiology. Wide range expression, stable expression level and high expression level are independent features of a single gene expression and are all desirable for the definition of an "ideal" HK. Recent studies have questioned the possible existence of "ideal" HK mRNAs, mainly because of the wide expression conditions variability. This would imply that for each investigated organism the suitability of a putative HK should be verified. We perform a systematic analysis to identify "optimal" HK genes in Danio rerio (zebrafish), to be used in expression analyses conducted on embryos/larvae at different developmental stages, as well as on differentiated adult tissues from single donors. The expression pattern of candidate genes, selected on the basis of the literature available and of ad hoc bioinformatics analysis, was assessed by quantitative relative RT-PCR in an RNA panel, including 6 different embryo/larvae developmental stages and 6 adult tissues. Statistical analysis was performed to identify genes with the lowest expression standard deviation in the studied panel. Our results showed that beta-actin 2 (bactin2) is the mRNA with the lowest variability of expression.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping