PUBLICATION
Cofilin regulator 14-3-3{zeta} is an evolutionarily conserved protein required for phagocytosis and microbial resistance
- Authors
- Ulvila, J., Vanha-Aho, L.M., Kleino, A., Vähä-Mäkilä, M., Vuoksio, M., Eskelinen, S., Hultmark, D., Kocks, C., Hallman, M., Parikka, M., and Rämet, M.
- ID
- ZDB-PUB-110110-33
- Date
- 2011
- Source
- Journal of Leukocyte Biology 89(5): 649-59 (Journal)
- Registered Authors
- Keywords
- Drosophila, zebrafish, plasmatocyte, macrophage, immunity
- MeSH Terms
-
- 14-3-3 Proteins/antagonists & inhibitors
- 14-3-3 Proteins/genetics*
- Actin Depolymerizing Factors/genetics*
- Animals
- Biological Evolution*
- Cells, Cultured
- Drosophila melanogaster/genetics*
- Drosophila melanogaster/immunology
- Drosophila melanogaster/microbiology*
- Escherichia coli/pathogenicity*
- Escherichia coli Infections/genetics
- Escherichia coli Infections/immunology
- Escherichia coli Infections/microbiology
- Female
- Gene Silencing
- Genes, Insect
- Hemocytes/metabolism
- Male
- Mice
- Phagocytosis/physiology*
- RNA, Small Interfering/genetics
- Zebrafish/genetics
- Zebrafish/immunology
- Zebrafish/microbiology
- PubMed
- 21208897 Full text @ J. Leukoc. Biol.
Citation
Ulvila, J., Vanha-Aho, L.M., Kleino, A., Vähä-Mäkilä, M., Vuoksio, M., Eskelinen, S., Hultmark, D., Kocks, C., Hallman, M., Parikka, M., and Rämet, M. (2011) Cofilin regulator 14-3-3{zeta} is an evolutionarily conserved protein required for phagocytosis and microbial resistance. Journal of Leukocyte Biology. 89(5):649-59.
Abstract
Phagocytosis is an ancient cellular process that plays an important role in host defense. In Drosophila melanogaster phagocytic, macrophage-like hemocytes recognize and ingest microbes. We performed an RNAi-based in vitro screen in the Drosophila hemocyte cell line S2 and identified Abi, cpa, cofilin regulator 14-3-3ζ, tlk, CG2765, and CG15609 as mediators of bacterial phagocytosis. Of these identified genes, 14-3-3ζ had an evolutionarily conserved role in phagocytosis: bacterial phagocytosis was compromised when 14-3-3ζ was targeted with RNAi in primary Drosophila hemocytes and when the orthologous genes Ywhab and Ywhaz were silenced in zebrafish and mouse RAW 264.7 cells, respectively. In Drosophila and zebrafish infection models, 14-3-3ζ was required for resistance against Staphylococcus aureus. We conclude that 14-3-3ζ is essential for phagocytosis and microbial resistance in insects and vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping