PUBLICATION

Role of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish

Authors
Geudens, I., Herpers, R., Hermans, K., Segura, I., Ruiz de Almodovar, C., Bussmann, J., De Smet, F., Vandevelde, W., Hogan, B.M., Siekmann, A., Claes, F., Moore, J.C., Pistocchi, A.S., Loges, S., Mazzone, M., Mariggi, G., Bruyere, F., Cotelli, F., Kerjaschki, D., Noel, A., Foidart, J.M., Gerhardt, H., Ny, A., Langenberg, T., Lawson, N.D., Duckers, H.J., Schulte-Merker, S., Carmeliet, P., and Dewerchin, M.
ID
ZDB-PUB-101213-1
Date
2010
Source
Arterioscler. Thromb. Vasc. Biol.   30(9): 1695-1702 (Journal)
Registered Authors
Bussmann, Jeroen, Cotelli, Franco, Herpers, Robert, Hogan, Ben M., Langenberg, Tobias, Lawson, Nathan, Moore, John, Schulte-Merker, Stefan, Siekmann, Arndt Friedrich
Keywords
vascular biology, gene silencing, lymphangiogenesis, notch signaling, zebrafish
MeSH Terms
  • Intracellular Signaling Peptides and Proteins
  • Coculture Techniques
  • COS Cells
  • Animals, Genetically Modified
  • Cell Proliferation
  • Gene Knockdown Techniques
  • Lymphatic System/embryology
  • Lymphatic System/metabolism*
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • RNA, Messenger/metabolism
  • Chlorocebus aethiops
  • Biomarkers/metabolism
  • Gene Silencing
  • Animals
  • Cell Movement
  • Endothelial Cells/metabolism
  • Luminescent Proteins/biosynthesis
  • Luminescent Proteins/genetics
  • Lymphangiogenesis*/genetics
  • Gene Expression Regulation, Developmental
  • Humans
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Receptors, Notch/genetics
  • Receptors, Notch/metabolism*
  • Signal Transduction*
  • Embryo, Nonmammalian/metabolism
  • Thoracic Duct/embryology
  • Thoracic Duct/metabolism
PubMed
20466977 Full text @ Arterioscler. Thromb. Vasc. Biol.
Abstract
OBJECTIVE: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. METHODS AND RESULTS: In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates. CONCLUSIONS: These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping