PUBLICATION
Chapter 10 - Molecular regulation of pancreas development in zebrafish
- Authors
- Kimmel, R.A., and Meyer, D.
- ID
- ZDB-PUB-101201-32
- Date
- 2010
- Source
- Methods in cell biology 100: 261-280 (Chapter)
- Registered Authors
- Kimmel, Robin, Meyer, Dirk
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Proliferation
- Insulin-Secreting Cells/cytology
- Insulin-Secreting Cells/physiology
- Pancreas/cytology
- Pancreas/embryology*
- Pancreas/physiology*
- Zebrafish/embryology*
- Zebrafish/physiology
- PubMed
- 21111221 Full text @ Meth. Cell. Biol.
Citation
Kimmel, R.A., and Meyer, D. (2010) Chapter 10 - Molecular regulation of pancreas development in zebrafish. Methods in cell biology. 100:261-280.
Abstract
The pancreas is a vertebrate-specific organ of endodermal origin which is responsible for production of digestive enzymes and hormones involved in regulating glucose homeostasis, in particular insulin, deficiency of which results in diabetes. Basic research on the genetic and molecular pathways regulating pancreas formation and function has gained major importance for the development of regenerative medical approaches aimed at improving diabetes treatment. Among the different model organisms that are currently used to elucidate the basic pathways of pancreas development and regeneration, the zebrafish is distinguished by its unique opportunities to combine genetic and pharmacological approaches with sophisticated live-imaging methodology, and by its ability to regenerate the pancreas within a short time. Here we review current perspectives and present methods for studying two important processes contributing to pancreas development and regeneration, namely cell migration via time-lapse micropscopy and cell proliferation via incorporation of nucleotide analog EdU, with a focus on the insulin-producing beta cells of the islet.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping