PUBLICATION

Identification and expression analysis of mical family genes in zebrafish

Authors
Xue, Y., Kuok, C., Xiao, A., Zhu, Z., Lin, S., and Zhang, B.
ID
ZDB-PUB-101108-3
Date
2010
Source
Journal of genetics and genomics = Yi chuan xue bao   37(10): 685-693 (Journal)
Registered Authors
Lin, Shuo, Zhu, Zuoyan
Keywords
zebrafish, mical, micall2a, expression pattern, blood vessel, heart
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Blood Vessels/embryology
  • Blood Vessels/metabolism
  • Cloning, Molecular
  • Cytoskeletal Proteins/genetics*
  • DNA, Complementary/chemistry
  • DNA, Complementary/genetics
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Profiling*
  • Gene Expression Regulation, Developmental
  • Heart/embryology
  • In Situ Hybridization
  • Intracellular Signaling Peptides and Proteins/genetics*
  • Molecular Sequence Data
  • Multigene Family/genetics*
  • Myocardium/metabolism
  • Phylogeny
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins/classification
  • Zebrafish Proteins/genetics*
PubMed
21035094 Full text @ J. Genet. Genomics
Abstract
Mical (molecule interacting with CasL) represent a conserved family of cytosolic multidomain proteins that has been shown to be associated with a variety of cellular processes, including axon guidance, cell movement, cell-cell junction formation, vesicle trafficking and cancer cell metastasis. However, the expression and function of these genes during embryonic development have not been comprehensively characterized, especially in vertebrate species, although some limited in vivo studies have been carried out in neural and musculature systems of Drosophila and in neural systems of vertebrates. So far, no mical family homologs have been reported in zebrafish, an ideal vertebrate model for the study of developmental processes. Here we report eight homologs of mical family genes in zebrafish and their expression profiles during embryonic development. Consistent with the findings in Drosophila and mammals, most zebrafish mical family genes display expression in neural and musculature systems. In addition, five mical homologs are detected in heart, and one, micall2a, in blood vessels. Our data established an important basis for further functional studies of mical family genes in zebrafish, and suggest a possible role for mical genes in cardiovascular development.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping