PUBLICATION
A cascade of irx1a and irx2a controls shh expression during retinogenesis
- Authors
- Choy, S.W., Cheng, C.W., Lee, S.T., Li, V.W., Hui, M.N., Hui, C.C., Liu, D., and Cheng, S.H.
- ID
- ZDB-PUB-101108-27
- Date
- 2010
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 239(12): 3204-3214 (Journal)
- Registered Authors
- Liu, Dong
- Keywords
- Iroquois, sonic hedgehog, Ath5, retina, retinal ganglion cells
- MeSH Terms
-
- Animals
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism
- Hedgehog Proteins/genetics
- Hedgehog Proteins/metabolism
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism*
- Retina/embryology*
- Retinal Ganglion Cells/cytology*
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 21046643 Full text @ Dev. Dyn.
Citation
Choy, S.W., Cheng, C.W., Lee, S.T., Li, V.W., Hui, M.N., Hui, C.C., Liu, D., and Cheng, S.H. (2010) A cascade of irx1a and irx2a controls shh expression during retinogenesis. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(12):3204-3214.
Abstract
In animal retina, hedgehog expression drives waves of neurogenesis, but genetic programs that control its expression during retinal neurogenesis are poorly elucidated. We have previously reported that irx1a is required for propagation of the sonic hedgehog (shh) expression waves in developing zebrafish retina. Here, we found that irx2a is expressed in the developing retina and that knockdown of irx2a results in a retinal phenotype strikingly similar to that of irx1a morphants. The expression of irx2a in retina ganglion cells was shown to be irx1a- and ath5-dependent suggesting that irx1a and ath5 are transcriptional regulators of irx2a. Furthermore, irx2a expression could rescue impaired propagation of shh waves in irx1a morphants. Together, these observations suggest that Irx2 functions downstream of irx1a to control shh expression in the retina. We proposed a novel transcriptional cascade of ath5-irx1a-irx2a in the regulation of hedgehog waves during vertebrate retinal development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping