PUBLICATION
Transgenic labeling of the zebrafish pronephric duct and tubules using a promoter from the enpep gene
- Authors
- Seiler, C., and Pack, M.
- ID
- ZDB-PUB-101027-34
- Date
- 2011
- Source
- Gene expression patterns : GEP 11(1-2): 118-121 (Journal)
- Registered Authors
- Pack, Michael, Seiler, Christoph
- Keywords
- zebrafish, pronephros, kidney, promoter, development, transgenic, glutamyl aminopeptidase, enpep, aminopeptidase A
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Embryo, Nonmammalian/metabolism
- Glutamyl Aminopeptidase/genetics*
- Kidney/embryology*
- Kidney/metabolism
- Kidney Tubules/embryology
- Kidney Tubules/metabolism
- Promoter Regions, Genetic*
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish/metabolism
- PubMed
- 20969977 Full text @ Gene Expr. Patterns
Citation
Seiler, C., and Pack, M. (2011) Transgenic labeling of the zebrafish pronephric duct and tubules using a promoter from the enpep gene. Gene expression patterns : GEP. 11(1-2):118-121.
Abstract
In recent years the zebrafish has become a popular model system to study organ development and disease. To facilitate these studies, genetic tools are required which allow to modify and manipulate gene expression in organs of interest. Here we describe a zebrafish 2 kb glutamyl aminopeptidase (enpep) promoter fragment, and show that it can drive gene expression specifically in the kidney during early and late development. We established a stable transgenic line using this promoter fragment that has specific GFP expression in pronephric ducts and tubules starting at 20 hours post-fertilization.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping