PUBLICATION
Chlamydomonas IFT70/CrDYF-1 Is a Core Component of IFT Particle Complex B and Is Required for Flagellar Assembly
- Authors
- Fan, Z.C., Behal, R.H., Geimer, S., Wang, Z., Williamson, S.M., Zhang, H., Cole, D.G., and Qin, H.
- ID
- ZDB-PUB-100614-31
- Date
- 2010
- Source
- Molecular biology of the cell 21(15): 2696-2706 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Algal Proteins/chemistry
- Algal Proteins/metabolism*
- Amino Acid Sequence
- Axoneme/metabolism
- Axoneme/ultrastructure
- Chlamydomonas/cytology
- Chlamydomonas/metabolism*
- Chlamydomonas/ultrastructure
- Conserved Sequence/genetics
- Flagella/metabolism*
- Flagella/ultrastructure
- Gene Knockdown Techniques
- Kinesins/metabolism
- Molecular Sequence Data
- Protein Binding
- Protein Subunits/metabolism
- Recombinant Proteins/metabolism
- PubMed
- 20534810 Full text @ Mol. Biol. Cell
Citation
Fan, Z.C., Behal, R.H., Geimer, S., Wang, Z., Williamson, S.M., Zhang, H., Cole, D.G., and Qin, H. (2010) Chlamydomonas IFT70/CrDYF-1 Is a Core Component of IFT Particle Complex B and Is Required for Flagellar Assembly. Molecular biology of the cell. 21(15):2696-2706.
Abstract
Monitoring Editor: Tim Stearns DYF-1 is a highly conserved protein essential for ciliogenesis in several model organisms. In Caenorhaditis elegans, DYF-1 serves as an essential activator for an anterograde motor OSM-3 of intraflagellar transport (IFT), the ciliogenesis-required motility process that mediates the transport of flagellar precursors and removal of turnover products. In zebrafish and Terahymena DYF-1 influences the cilia tubulin post-translational modification and may have more ubiquitous function in ciliogenesis than OSM-3. Here we address how DYF-1 biochemically interacts with the IFT machinery by using the model organism Chlamydomonas reinhardtii, in which the anterograde IFT does not depend on OSM-3. Our results show that this protein is a stoichiometric component of the IFT particle complex B and interacts directly with complex B subunit IFT46. In concurrence with the established IFT protein nomenclature, DYF-1 is also named IFT70 after the apparent size of the protein. IFT70/CrDYF-1 is essential for the function of IFT in building the flagellum since the flagella of IFT70/CrDYF-1 depleted cells were greatly shortened. Together, these results demonstrate that IFT70/CrDYF-1 is a canonical subunit of IFT particle complex B, and strongly support the hypothesis that the IFT machinery has species- and tissue- specific variations with functional ramifications.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
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Orthology
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