PUBLICATION
Tandem Alternative Splicing of Zebrafish connexin45.6
- Authors
- Tao, L., and Valdimarsson, G.
- ID
- ZDB-PUB-100518-13
- Date
- 2010
- Source
- Genomics 96(2): 112-118 (Journal)
- Registered Authors
- Valdimarsson, Gunnar
- Keywords
- Connexin, Gap junction, Tandem alternative splicing, Alternative promoter, Genomic structure, Zebrafish
- MeSH Terms
-
- 5' Untranslated Regions/genetics
- Alternative Splicing/genetics*
- Animals
- Base Sequence
- Connexins/genetics*
- Connexins/metabolism
- DNA Primers/genetics
- Gene Components
- Gene Expression Regulation/genetics*
- Molecular Sequence Data
- Protein Isoforms/genetics
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Analysis, DNA
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 20466054 Full text @ Genomics
Citation
Tao, L., and Valdimarsson, G. (2010) Tandem Alternative Splicing of Zebrafish connexin45.6. Genomics. 96(2):112-118.
Abstract
Early studies suggested that most connexin genes share a relatively simple structure with a single intron of variable length interrupting the 5' untranslated region (UTR). Here we report that zebrafish cx45.6 shows six isoforms of alternative 5'UTRs which are generated from multiple promoter usage and alternative pre-mRNA splicing. Interestingly, cx45.6 undergoes tandem alternative splicing, which produces transcripts only differing by 3 nucleotides. This is the first study that has demonstrated tandem alternative pre-mRNA splicing in the connexin gene family. Expression patterns of cx45.6 alternative transcripts were demonstrated by real-time RT-PCR during zebrafish embryonic development and in adult tissues. The complexity of 5'UTR diversity suggests complicated regulatory mechanisms for cx45.6 gene expression at both transcriptional and post-transcriptional levels, and we propose that tandem alternative splicing in cx45.6 5'UTRs could play a role in translational control. These results lay groundwork for further investigations on the regulation and function of cx45.6 gene expression.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping