PUBLICATION
GH overexpression modifies muscle expression of anti-oxidant enzymes and increases spinal curvature of old zebrafish
- Authors
- Rosa, C.E., Kuradomi, R.Y., Almeida, D.V., Lannes, C.F., de Azevedo Figueiredo, M., Dytz, A.G., Fonseca, D.B., and Marins, L.F.
- ID
- ZDB-PUB-100330-27
- Date
- 2010
- Source
- Experimental gerontology 45(6): 449-456 (Journal)
- Registered Authors
- Almeida, Daniela Volcan, da Rosa, Carlos Eduardo, Kuradomi, Rafael, Marins, Luis Fernando
- Keywords
- Growth hormone, Aging, Anti-oxidant enzymes, Spinal curvature, Myogenic factors, Lipofuscin, Growth curve
- MeSH Terms
-
- Aging/physiology
- Animals
- Animals, Genetically Modified/genetics
- Animals, Genetically Modified/metabolism
- Antioxidants/metabolism
- Carps/genetics
- Gene Amplification
- Gene Expression Regulation, Developmental
- Glutamate-Cysteine Ligase/genetics*
- Glutathione/genetics
- Glutathione/metabolism
- Growth Charts
- Growth Hormone/genetics
- Growth Hormone/metabolism*
- Growth Hormone/physiology
- Lipid Peroxidation/genetics
- Lipofuscin
- Male
- Muscle Development/genetics*
- Muscle, Skeletal/metabolism
- Phenotype
- Polymerase Chain Reaction
- Protein Isoforms/genetics
- Protein Isoforms/metabolism
- RNA, Messenger/metabolism
- Spinal Curvatures/metabolism*
- Superoxide Dismutase/genetics*
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 20338232 Full text @ Exp. Gerontol.
Citation
Rosa, C.E., Kuradomi, R.Y., Almeida, D.V., Lannes, C.F., de Azevedo Figueiredo, M., Dytz, A.G., Fonseca, D.B., and Marins, L.F. (2010) GH overexpression modifies muscle expression of anti-oxidant enzymes and increases spinal curvature of old zebrafish. Experimental gerontology. 45(6):449-456.
Abstract
Growth hormone (GH) excess causes an increment in the metabolic rate and in reactive oxygen species generation, which accelerate the ageing process in mammals. Considering that there is no information on this subject in fish, the aim of the present study was to evaluate the excess GH effect on senescence in a zebrafish (Danio rerio) transgenic model. In order to reach this objective, we analyzed the phenotype of spinal curvature and expression of genes related to the antioxidant defense system and myogenesis in muscle of 8 and 30 months old GH-transgenic males. Gene expression analyses revealed that both superoxide dismutase isoforms were down-regulated only in 30 months old animals, while glutamate cysteine ligase was down-regulated in GH-transgenic zebrafish. It was also observed an acceleration of the spinal curvature and a reduction in the expression of miogenin in both ages and MyoD in the old ones. Even neurolipofuscin accumulation was not significant in GH-transgenic zebrafish, the estimation of maximum longevity based on the von Bertalanffy growth function was significantly lower in this group. The results obtained here indicate that GH overexpression reduces the transcription of antioxidant defense system and myogenesis-related genes, which probably accelerates senescence in the zebrafish transgenic model used.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping