PUBLICATION
Transgenic Labeling of Hair Cells in the Zebrafish Acousticolateralis System
- Authors
- McDermott, B.M., Asai, Y., Baucom, J.M., Jani, S.D., Castellanos, Y., Gomez, G., McClintock, J.M., and Hudspeth, A.J.
- ID
- ZDB-PUB-100126-3
- Date
- 2010
- Source
- Gene expression patterns : GEP 10(2-3): 113-118 (Journal)
- Registered Authors
- Asai, Yukako, Gomez, Gustavo, Hudspeth, A.J. (Jim), McClintock, James, McDermott Jr., Brian M.
- Keywords
- Balance, Hearing, Lateral line, Mantle cell, Parvalbumin, Supporting cell
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental
- Green Fluorescent Proteins/genetics
- Hair Cells, Auditory/metabolism*
- Parvalbumins/genetics*
- Transgenes*
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish Proteins/genetics*
- PubMed
- 20085825 Full text @ Gene Expr. Patterns
Citation
McDermott, B.M., Asai, Y., Baucom, J.M., Jani, S.D., Castellanos, Y., Gomez, G., McClintock, J.M., and Hudspeth, A.J. (2010) Transgenic Labeling of Hair Cells in the Zebrafish Acousticolateralis System. Gene expression patterns : GEP. 10(2-3):113-118.
Abstract
The zebrafish provides a useful experimental system for investigations of aural development. To permit the controlled expression of transgenes in developing hair cells, we isolated the genomic control regions of the parvalbumin3a (pvalb3a) and parvalbumin3b (pvalb3b) genes. Deletion analysis and somatic-cell transgenesis restricted the cis-acting control regions for hair cells to as little as 484base pairs for pvalb3a and 650base pairs for pvalb3b. Using both meganuclease-mediated and standard methods, we produced transgenic animals that transmit transgenes through their germ lines. These fish express GFP in hair cells in the inner ear and lateral line. Two stable transgenic lines express GFP prior to hair-bundle formation, so the associated promoter constructs are suitable for manipulating gene expression during bundle development. We additionally identified a transgenic line that offers variable labeling of supporting cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping