PUBLICATION
DeltaA mRNA and protein distribution in the zebrafish nervous system
- Authors
- Tallafuss, A., Trepman, A., and Eisen, J.S.
- ID
- ZDB-PUB-091120-76
- Date
- 2009
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 238(12): 3226-3236 (Journal)
- Registered Authors
- Eisen, Judith S., Tallafuss, Alexandra
- Keywords
- notch, proliferative zone, neurogenesis, differentiation, stem cell, antibody
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Proliferation
- Embryo, Nonmammalian
- Gene Expression Regulation, Developmental
- Intracellular Signaling Peptides and Proteins
- Membrane Proteins/genetics*
- Membrane Proteins/metabolism*
- Models, Biological
- Nervous System/embryology
- Nervous System/metabolism*
- Neurons/metabolism
- RNA, Messenger/metabolism
- Stem Cells/metabolism
- Tissue Distribution
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- PubMed
- 19924821 Full text @ Dev. Dyn.
Citation
Tallafuss, A., Trepman, A., and Eisen, J.S. (2009) DeltaA mRNA and protein distribution in the zebrafish nervous system. Developmental Dynamics : an official publication of the American Association of Anatomists. 238(12):3226-3236.
Abstract
Physical interaction between the transmembrane proteins Delta and Notch allows only a subset of neural precursors to become neurons, as well as regulating other aspects of neural development. To examine the localization of Delta protein during neural development, we generated an antibody specific to zebrafish DeltaA (Dla). Here, we describe for the first time the subcellular localization of Dla protein in distinct puncta at cell cortex and/or membrane, supporting the function of Dla in direct cell-cell communication. In situ RNA hybridization and immunohistochemistry revealed dynamic, coordinated expression patterns of dla mRNA and Dla protein in the developing and adult zebrafish nervous system. Dla expression is mostly excluded from differentiated neurons and is maintained in putative precursor cells at least until larval stages. In the adult brain, dla mRNA and Dla protein are expressed in proliferative zones normally associated with stem cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping