PUBLICATION
Normal table of postembryonic zebrafish development: Staging by externally visible anatomy of the living fish
- Authors
- Parichy, D.M., Elizondo, M.R., Mills, M.G., Gordon, T.N., and Engeszer, R.E.
- ID
- ZDB-PUB-091120-25
- Date
- 2009
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 238(12): 2975-3015 (Journal)
- Registered Authors
- Elizondo, Michael, Engeszer, Ray, Parichy, David M.
- Keywords
- Danio rerio, zebrafish, normal stages, skeleton, swim bladder, flexion, pigment pattern, fin, fin fold
- MeSH Terms
-
- Animals
- Body Weights and Measures/methods
- Bone Development/physiology
- Bone and Bones/physiology
- Embryo, Nonmammalian
- Extremities/physiology
- Health Planning Guidelines
- Life Cycle Stages/physiology
- Phenotype
- Skin Pigmentation/physiology
- Statistics as Topic*
- Zebrafish/anatomy & histology*
- Zebrafish/embryology
- Zebrafish/growth & development*
- Zebrafish/physiology
- PubMed
- 19891001 Full text @ Dev. Dyn.
Citation
Parichy, D.M., Elizondo, M.R., Mills, M.G., Gordon, T.N., and Engeszer, R.E. (2009) Normal table of postembryonic zebrafish development: Staging by externally visible anatomy of the living fish. Developmental Dynamics : an official publication of the American Association of Anatomists. 238(12):2975-3015.
Abstract
The zebrafish is a premier model organism yet lacks a system for assigning postembryonic fish to developmental stages. To provide such a staging series, we describe postembryonic changes in several traits that are visible under brightfield illumination or through vital staining and epiflourescent illumination. These include the swim bladder, median and pelvic fins, pigment pattern, scale formation, larval fin fold, and skeleton. We further identify milestones for placing postembryonic fish into discrete stages. We relate these milestones to changes in size and age and show that size is a better indicator of developmental progress than is age. We also examine how relationships between size and developmental progress vary with temperature and density, and we document the effects of histological processing on size. To facilitate postembryonic staging, we provide images of reference individuals that have attained specific developmental milestones and are of defined sizes. Finally, we provide guidelines for reporting stages that provide information on both discrete and continuous changes in growth and development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping