PUBLICATION
Anosmin-1a is required for fasciculation and terminal targeting of olfactory sensory neuron axons in the zebrafish olfactory system
- Authors
- Yanicostas, C., Herbomel, E., Dipietromaria, A., and Soussi-Yanicostas, N.
- ID
- ZDB-PUB-090526-33
- Date
- 2009
- Source
- Molecular and Cellular Endocrinology 312(1-2): 53-60 (Journal)
- Registered Authors
- Soussi-Yanicostas, Nadia
- Keywords
- Anosmin-1, Kallmann syndrome, Olfactory system, Zebrafish, kal1a gene
- MeSH Terms
-
- Gene Expression Regulation, Developmental
- Olfactory Bulb/embryology*
- Olfactory Bulb/metabolism
- Olfactory Bulb/pathology
- In Situ Hybridization, Fluorescence
- Brain/embryology
- Brain/metabolism
- Brain/pathology
- Neurogenesis/genetics
- Axons/metabolism*
- Nerve Tissue Proteins/deficiency
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism
- Nerve Tissue Proteins/physiology*
- Animals
- Time Factors
- Olfactory Receptor Neurons/embryology*
- Olfactory Receptor Neurons/metabolism
- Olfactory Mucosa/embryology
- Olfactory Mucosa/metabolism
- Olfactory Mucosa/pathology
- Sensory Receptor Cells/classification
- Sensory Receptor Cells/metabolism
- Sensory Receptor Cells/pathology
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism
- Olfactory Pathways/embryology
- Olfactory Pathways/metabolism
- Animals, Genetically Modified/embryology
- Animals, Genetically Modified/metabolism
- Image Processing, Computer-Assisted
- Interneurons/metabolism
- Interneurons/pathology
- Embryo, Nonmammalian/innervation
- Embryo, Nonmammalian/metabolism
- Oligodeoxyribonucleotides, Antisense/metabolism
- Protein Transport
- Zebrafish Proteins/deficiency
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish Proteins/physiology*
- PubMed
- 19464344 Full text @ Mol. Cell. Endocrinol.
Citation
Yanicostas, C., Herbomel, E., Dipietromaria, A., and Soussi-Yanicostas, N. (2009) Anosmin-1a is required for fasciculation and terminal targeting of olfactory sensory neuron axons in the zebrafish olfactory system. Molecular and Cellular Endocrinology. 312(1-2):53-60.
Abstract
The KAL-1 gene underlies the X-linked form of Kallmann syndrome (KS), a neurological disorder that impairs the development of the olfactory and GnRH systems. KAL-1 encodes anosmin-1, a cell matrix protein that shows cell adhesion, neurite outgrowth, and axon-guidance and -branching activities. We used zebrafish embryos as model to better understand the role of this protein during olfactory system (OS) development. First, we detected the protein in olfactory sensory neurons from 22hours post-fertilization (hpf) onward, i.e. prior their pioneer axons reached presumptive olfactory bulbs (OBs). We found that anosmin-1a depletion impaired the fasciculation of olfactory axons and their terminal targeting within OBs. Last, we showed that kal1a inactivation induced a severe decrease in the number of GABAergic and dopaminergic OB neurons. Though the phenotypes induced following anosmin-1a depletion in zebrafish embryos did not match precisely the defects observed in KS patients, our results provide the first demonstration of a direct requirement for anosmin-1 in OS development in vertebrates and stress the role of OB innervation on OB neuron differentiation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping