PUBLICATION
Alternative splicing, phylogenetic analysis, and craniofacial expression of zebrafish tbx22
- Authors
- Jezewski, P.A., Fang, P.K., Payne-Ferreira, T.L., and Yelick, P.C.
- ID
- ZDB-PUB-090511-10
- Date
- 2009
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 238(6): 1605-1612 (Journal)
- Registered Authors
- Fang, Pingke, Jezewski, Peter, Payne-Ferreira, Tracie, Yelick, Pamela C.
- Keywords
- zebrafish, craniofacial development, tbx22
- MeSH Terms
-
- Alternative Splicing*
- Amino Acid Sequence
- Animals
- Base Sequence
- Body Patterning/genetics
- Craniofacial Abnormalities/genetics
- Facial Bones/anatomy & histology
- Facial Bones/embryology*
- Facial Bones/physiology
- Gene Expression Regulation, Developmental
- Humans
- Molecular Sequence Data
- Phylogeny
- Protein Isoforms/genetics
- Protein Isoforms/metabolism
- Sequence Alignment
- Skull/anatomy & histology
- Skull/embryology*
- Skull/physiology
- T-Box Domain Proteins/chemistry
- T-Box Domain Proteins/classification
- T-Box Domain Proteins/genetics*
- T-Box Domain Proteins/metabolism*
- Zebrafish/anatomy & histology
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/physiology
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism*
- PubMed
- 19418442 Full text @ Dev. Dyn.
Citation
Jezewski, P.A., Fang, P.K., Payne-Ferreira, T.L., and Yelick, P.C. (2009) Alternative splicing, phylogenetic analysis, and craniofacial expression of zebrafish tbx22. Developmental Dynamics : an official publication of the American Association of Anatomists. 238(6):1605-1612.
Abstract
Mutations in human TBX22 cause X-linked cleft palate with ankyloglossia syndrome (CPX; OMIM 303400). Since the secondary palate was an adaptation to breathing on land, we characterized zebrafish tbx22 to study molecular mechanisms regulating early vertebrate craniofacial patterning. Rapid Amplification of cDNA Ends (RACE) analyses revealed two zebrafish tbx22 splice isoforms, tbx22-1 and tbx22-2, encoding proteins of 444 and 400 amino acids, respectively. tbx22-1 resembles canonical Tbx22 orthologs, while tbx22-2 lacks conserved N-terminal sequence. Developmental RT-PCR revealed that tbx22-1 is maternally and zygotically expressed, while tbx22-2 is expressed zygotically. WISH analyses revealed strong tbx22 mRNA expression in ectomesenchyme underlying the stomodeum, a bilaminar epithelial structure demarcating early mouth formation, and in early presumptive jaw joints. Zebrafish tbx22 expression mirrored some aspects of mammalian Tbx22, consistent with roles in early vertebrate face patterning. These studies identify an early transcription factor governing vertebrate facial development, which may underlie common craniofacial birth disorders.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping