PUBLICATION
Ontogeny and nutritional control of adipogenesis in zebrafish (Danio rerio)
- Authors
- Flynn, E.J. 3rd, Trent, C.M., and Rawls, J.F.
- ID
- ZDB-PUB-090417-9
- Date
- 2009
- Source
- Journal of Lipid Research 50(8): 1641-1652 (Journal)
- Registered Authors
- Flynn, Edward J., Rawls, John F., Trent, Chad
- Keywords
- none
- MeSH Terms
-
- Adipocytes/physiology*
- Adipocytes/ultrastructure
- Adipogenesis/physiology*
- Animals
- Body Composition
- Body Fat Distribution
- Body Weight
- Fatty Acid-Binding Proteins/genetics
- Fluorescent Dyes
- Food*
- Food Deprivation/physiology
- Gene Expression
- Heart/growth & development
- Hematopoietic System/growth & development
- Intra-Abdominal Fat/embryology
- Intra-Abdominal Fat/growth & development*
- Lipids/analysis
- Lipids/physiology*
- Models, Animal*
- Nutritional Status
- Oxazines
- PPAR gamma/genetics
- Pancreas/growth & development
- RNA, Messenger/analysis
- Whole Body Imaging/methods
- Zebrafish/anatomy & histology
- Zebrafish/embryology
- Zebrafish/growth & development
- Zebrafish/physiology*
- Zebrafish Proteins/genetics
- PubMed
- 19366995 Full text @ J. Lipid Res.
Citation
Flynn, E.J. 3rd, Trent, C.M., and Rawls, J.F. (2009) Ontogeny and nutritional control of adipogenesis in zebrafish (Danio rerio). Journal of Lipid Research. 50(8):1641-1652.
Abstract
The global obesity epidemic demands an improved understanding of the developmental and environmental factors regulating fat storage. Adipocytes serve as major sites of fat storage and as regulators of energy balance and inflammation. The optical transparency of developing zebrafish provides new opportunities to investigate mechanisms governing adipocyte biology, however zebrafish adipocytes remain uncharacterized. We have developed methods for visualizing zebrafish adipocytes in vivo by labeling neutral lipid droplets with Nile Red. Our results establish that neutral lipid droplets first accumulate in visceral adipocytes during larval stages, and increase in number and distribution as zebrafish grow. We show that the cellular anatomy of zebrafish adipocytes is similar to mammalian white adipocytes, and identify peroxisome-proliferator activated receptor gamma (pparg) and fatty acid-binding protein 11a (fabp11a) as markers of the zebrafish adipocyte lineage. By monitoring adipocyte development prior to neutral lipid deposition, we find that the first visceral preadipocytes appear in association with the pancreas shortly after initiation of exogenous nutrition. Zebrafish reared in the absence of food fail to form visceral preadipocytes, indicating that exogenous nutrition is required for adipocyte development. These results reveal homologies between zebrafish and mammalian adipocytes, and establish the zebrafish as a new model for adipocyte research.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping