PUBLICATION
Lin9 is required for mitosis and cell survival during early zebrafish development
- Authors
- Kleinschmidt, M.A., Wagner, T.U., Liedtke, D., Spahr, S., Samans, B., and Gaubatz, S.
- ID
- ZDB-PUB-090318-10
- Date
- 2009
- Source
- The Journal of biological chemistry 284(19): 13119-13127 (Journal)
- Registered Authors
- Liedtke, Daniel, Wagner, Toni
- Keywords
- none
- MeSH Terms
-
- Oligonucleotide Array Sequence Analysis
- Zebrafish
- Gene Expression Profiling
- Flow Cytometry
- Animals
- Morpholines/pharmacology
- Mitosis/physiology*
- Apoptosis
- Embryonic Development/physiology*
- Embryo, Nonmammalian/physiology*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- In Situ Hybridization
- Cell Survival/physiology
- Cell Cycle Proteins/genetics*
- Cell Cycle Proteins/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish Proteins/physiology*
- Gene Expression Regulation, Developmental*
- Reverse Transcriptase Polymerase Chain Reaction
- PubMed
- 19278998 Full text @ J. Biol. Chem.
Citation
Kleinschmidt, M.A., Wagner, T.U., Liedtke, D., Spahr, S., Samans, B., and Gaubatz, S. (2009) Lin9 is required for mitosis and cell survival during early zebrafish development. The Journal of biological chemistry. 284(19):13119-13127.
Abstract
LIN9 has been described as a regulator of G1/S and G2/M progression of the cell cycle in invertebrates and human cell lines. To elucidate the in vivo function of LIN9 during vertebrate development, we took advantage of the teleost zebrafish (Danio rerio). By means of antisense morpholinos we show here that Lin9-depleted embryonic cells accumulate in mitosis. Flow cytometry and confocal microscopy data demonstrate that the delay in mitotic progression is followed by apoptosis, which strongly manifests in the developing CNS. In accordance with these findings we identified a cohort of Lin9 regulated genes required for different mitotic processes, including mitotic entry, metaphase/anaphase transition and cytokinesis. Our data establish LIN9 as an essential regulator of mitosis in vertebrate development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping