PUBLICATION
Comparative Analysis Of Gene Expression In Brain, Liver, Skeletal Muscles And Gills Of The Zebrafish (Danio rerio) Exposed To Environmentally Relevant Waterborne Uranium Concentrations
- Authors
- Lerebours, A., Gonzalez, P., Adam, C., Camilleri, V., Bourdineaud, J.P., and Garnier-Laplace, J.
- ID
- ZDB-PUB-090123-3
- Date
- 2009
- Source
- Environmental toxicology and chemistry 28(6): 1271-1278 (Journal)
- Registered Authors
- Keywords
- Uranium, Danio rerio, Gene expression, Bioaccumulation
- MeSH Terms
-
- Animals
- Base Sequence
- Brain/drug effects*
- Brain/metabolism
- DNA Primers
- Gene Expression/drug effects*
- Gills/drug effects*
- Gills/metabolism
- Liver/drug effects*
- Liver/metabolism
- Muscle, Skeletal/drug effects*
- Muscle, Skeletal/metabolism
- Polymerase Chain Reaction
- Uranium/toxicity*
- Water Pollutants, Radioactive/toxicity*
- Zebrafish
- PubMed
- 19146232 Full text @ Environ. Toxicol. Chem.
Citation
Lerebours, A., Gonzalez, P., Adam, C., Camilleri, V., Bourdineaud, J.P., and Garnier-Laplace, J. (2009) Comparative Analysis Of Gene Expression In Brain, Liver, Skeletal Muscles And Gills Of The Zebrafish (Danio rerio) Exposed To Environmentally Relevant Waterborne Uranium Concentrations. Environmental toxicology and chemistry. 28(6):1271-1278.
Abstract
The effects of waterborne uranium (U) exposure on gene expression were examined in four organs (brain, liver, skeletal muscles and gills) of the zebrafish (Danio rerio). Adult male fish were exposed to three exposure treatments: no added U, 23 +/- 6 and 130 +/- 34 microg U/L. After 3, 10, 21, and 28 d of exposure and an 8-d depuration period, gene expression and U bioaccumulation were analyzed. Bioaccumulation decreased significantly in liver during the depuration phase, and genes involved in detoxification, apoptotic mechanism and immune response were strongly induced. Among these genes, abcb3l1, which belongs to the ABC transporter family, was 4-times and 24-times induced in organisms previously exposed to 23 +/- and 130 +/- microg/L of U, respectively. These results highlight the role of liver in detoxification mechanisms. In gills, at the highest U concentration, gpx1a, cat, sod1, and sod2 genes were up-regulated at day 21, indicating the onset of an oxidative stress. Mitochondrial metabolism and DNA integrity were also affected since coxI, atp5f1, and rad51 genes were up-regulated at day 21 and during the depuration phase. In skeletal muscles, coxI, atp5f1, and cat were induced at day 3 suggesting an impact on the mitochondrial metabolism and production of reactive oxygen species. In brain, glsI was also induced at day 3 suggesting a need in glutamate synthesis involved in neuron transmission. No changes in gene expression were observed in brain and skeletal muscles at days 21 and 28 of exposure although bioaccumulation increased. During the depuration phase, U excretion was inefficient in brain and skeletal muscles, and expression of most of the tissue-specific genes was repressed or unchanged.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping