PUBLICATION
Zebrafish neurolin-a and -b, orthologs of ALCAM, are involved in retinal ganglion cell differentiation and retinal axon pathfinding
- Authors
- Diekmann, H., and Stuermer, C.A.
- ID
- ZDB-PUB-090106-20
- Date
- 2009
- Source
- The Journal of comparative neurology 513(1): 38-50 (Journal)
- Registered Authors
- Diekmann, Heike
- Keywords
- zebrafish retinotectal system, morpholino knockdown, gene duplication
- MeSH Terms
-
- Activated-Leukocyte Cell Adhesion Molecule/classification
- Activated-Leukocyte Cell Adhesion Molecule/genetics
- Activated-Leukocyte Cell Adhesion Molecule/metabolism*
- Animals
- Animals, Genetically Modified
- Antigens, CD/genetics
- Antigens, CD/metabolism
- Axons/physiology*
- Cell Adhesion Molecules, Neuronal/genetics
- Cell Adhesion Molecules, Neuronal/metabolism
- Cell Differentiation/physiology*
- Cell Movement/physiology
- Eye/anatomy & histology
- Fetal Proteins/genetics
- Fetal Proteins/metabolism
- Humans
- In Situ Hybridization
- Oligonucleotides, Antisense
- Phenotype
- Phylogeny
- Protein Isoforms/genetics
- Protein Isoforms/metabolism*
- Retina/abnormalities
- Retina/cytology
- Retina/embryology
- Retina/growth & development
- Retinal Ganglion Cells/cytology
- Retinal Ganglion Cells/physiology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 19107846 Full text @ J. Comp. Neurol.
Citation
Diekmann, H., and Stuermer, C.A. (2009) Zebrafish neurolin-a and -b, orthologs of ALCAM, are involved in retinal ganglion cell differentiation and retinal axon pathfinding. The Journal of comparative neurology. 513(1):38-50.
Abstract
Neurolin-a and Neurolin-b (also called alcam and nlcam, respectively) are zebrafish orthologs of human ALCAM, an adhesion protein of the immunoglobulin superfamily with functions in axon growth and guidance. Within the developing zebrafish retina, onset and progression of Neurolin-a expression parallels the pattern of retinal ganglion cell (RGC) differentiation. By using a morpholino-based knockdown approach, we show that Neurolin-a (but not Neurolin-b) is necessary for a crucial step in RGC differentiation. Without Neurolin-a, a large proportion of RGCs fail to develop, and RGC axons are absent or reduced in number. Subsequently, Neurolin-a is required for RGC survival and for the differentiation of all other retinal neurons. Neurolin-b is expressed later in well-differentiated RGCs and is required for RGC axon pathfinding. Without Neurolin-b, RGC axons grow in highly aberrant routes along the optic tract and/or fail to reach the optic tectum. Thus, the zebrafish Neurolin paralogs are involved in distinct steps of retinotectal development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping