PUBLICATION
The Sphingolipid Transporter Spns2 Functions in Migration of Zebrafish Myocardial Precursors
- Authors
- Kawahara, A., Nishi, T., Hisano, Y., Fukui, H., Yamaguchi, A., and Mochizuki, N.
- ID
- ZDB-PUB-081218-20
- Date
- 2009
- Source
- Science (New York, N.Y.) 323(5913): 524-527 (Journal)
- Registered Authors
- Kawahara, Atsuo
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Blastomeres/metabolism
- CHO Cells
- Carrier Proteins/chemistry
- Carrier Proteins/genetics
- Carrier Proteins/metabolism*
- Cell Movement
- Cricetinae
- Cricetulus
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism*
- Embryonic Development
- Heart/embryology*
- Heart Defects, Congenital/embryology
- Humans
- Lysophospholipids/metabolism*
- Membrane Proteins/chemistry
- Membrane Proteins/genetics
- Membrane Proteins/metabolism*
- Mesoderm/metabolism
- Mice
- Molecular Sequence Data
- Mutation
- Oligonucleotides, Antisense
- Organogenesis
- Signal Transduction
- Somites/embryology
- Somites/metabolism
- Sphingosine/analogs & derivatives*
- Sphingosine/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 19074308 Full text @ Science
Citation
Kawahara, A., Nishi, T., Hisano, Y., Fukui, H., Yamaguchi, A., and Mochizuki, N. (2009) The Sphingolipid Transporter Spns2 Functions in Migration of Zebrafish Myocardial Precursors. Science (New York, N.Y.). 323(5913):524-527.
Abstract
Sphingosine-1-phosphate (S1P) is a secreted lipid mediator that functions in vascular development: however, it remains unclear how S1P secretion is regulated during embryogenesis. We identified a zebrafish mutant ko157, which displays cardia bifida (two hearts) resembling the S1P receptor-2 mutant. A migration defect of myocardial precursors in the ko157 mutant is due to a mutation in a multipass transmembrane protein Spns2 and can be rescued by S1P injection. We show that the export of S1P from cells requires Spns2. spns2 is expressed in the extra-embryonic tissue yolk syncytial layer (YSL) and introduction of spns2 mRNA in the YSL restored the cardiac defect in the ko157 mutant. Thus, Spns2 in the YSL functions as a S1P transporter in S1P secretion, thereby regulating myocardial precursor migration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping