PUBLICATION
Identification and expression pattern of zebrafish prox2 during embryonic development
- Authors
- Pistocchi, A., Bartesaghi, S., Cotelli, F., and Del Giacco, L.
- ID
- ZDB-PUB-081203-19
- Date
- 2008
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 237(12): 3916-3920 (Journal)
- Registered Authors
- Cotelli, Franco, Del Giacco, Luca
- Keywords
- prospero, homeodomain, prox1, prox2, zebrafish, whole-mount in situ hybridization, RT-PCR, embryonic development
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- DNA, Complementary/genetics
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Embryonic Development*
- Gene Expression Regulation, Developmental*
- Homeodomain Proteins/chemistry
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism*
- Humans
- Molecular Sequence Data
- Phylogeny
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 19035352 Full text @ Dev. Dyn.
Citation
Pistocchi, A., Bartesaghi, S., Cotelli, F., and Del Giacco, L. (2008) Identification and expression pattern of zebrafish prox2 during embryonic development. Developmental Dynamics : an official publication of the American Association of Anatomists. 237(12):3916-3920.
Abstract
Prox2, together with the previously isolated Prox1, is the vertebrate homolog of the Drosophila homeobox-containing gene prospero, the founder member of a family of transcription factors which have been shown to play critical roles in many developmental events. We have isolated a cDNA which encodes a putative protein that shares a high degree of homology with mammalian Prox1, Prox2, and zebrafish Prox1. Comparative genomic analysis revealed that this protein corresponds to the zebrafish Prox2 homolog being the gene syntenic with the chromosome region hosting mouse Prox2. Whole-mount in situ experiments demonstrated that prox2 is expressed, during zebrafish embryonic development, in defined structures of the central nervous system and the eye, as previously reported in mouse. Additionally, reverse transcriptase-polymerase chain reaction analysis disclosed prox2 expression in several adult organs. Finally, prox1 loss- and gain-of-function assays have been carried out to search for regulative effects on prox2 expression.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping