PUBLICATION
Antiangiogenic activity of Tripterygium wilfordii and its terpenoids
- Authors
- He, M.F., Liu, L., Ge, W., Shaw, P.C., Jiang, R., Wu, L.W., and But, P.P.
- ID
- ZDB-PUB-081114-5
- Date
- 2009
- Source
- Journal of ethnopharmacology 121(1): 61-68 (Journal)
- Registered Authors
- Ge, Wei
- Keywords
- Antiangiogenesis, Zebrafish, Tripterygium wilfordii, Triptolide, Angpt2, Tie2
- MeSH Terms
-
- Angiogenesis Inhibitors/chemistry
- Angiogenesis Inhibitors/pharmacology*
- Animals
- Disease Models, Animal
- Diterpenes/chemistry
- Diterpenes/pharmacology
- Drugs, Chinese Herbal/pharmacology
- Embryo, Nonmammalian/blood supply
- Epoxy Compounds/chemistry
- Epoxy Compounds/pharmacology
- Neovascularization, Physiologic/drug effects
- Phenanthrenes/chemistry
- Phenanthrenes/pharmacology
- Plant Roots/chemistry
- Reverse Transcriptase Polymerase Chain Reaction
- Terpenes/chemistry
- Terpenes/pharmacology*
- Tripterygium/chemistry*
- Triterpenes/chemistry
- Triterpenes/pharmacology
- Zebrafish
- PubMed
- 18996177 Full text @ J. Ethnopharmacol.
Citation
He, M.F., Liu, L., Ge, W., Shaw, P.C., Jiang, R., Wu, L.W., and But, P.P. (2009) Antiangiogenic activity of Tripterygium wilfordii and its terpenoids. Journal of ethnopharmacology. 121(1):61-68.
Abstract
Tripterygium wilfordii Hook. f. (Celastraceae) has been traditionally used as folk medicine for centuries in China for the treatment of immune-inflammatory diseases. This study aimed to assess the antiangiogenic activities which support the therapeutic use of Tripterygium wilfordii and its terpenoids for angiogenesis disease such as cancer. The ethanol extract of Tripterygium wilfordii and subsequent fractions were evaluated on an in vivo antiangiogenic zebrafish embryo model. Three antiangiogenic terpenoids were isolated by bioassay-guided purification, namely, celastrol (4), cangoronine (5) and triptolide (7). Among them, triptolide manifested the most potent antiangiogenic activity against vessel formation by nearly 50% at 1.2muM. Semi-quantitative RT-PCR analysis revealed that triptolide dose- and time-dependently reduced the mRNA expression of angiopoietin (angpt)2 and tie2 in zebrafish, indicating the involvement of angpt2/tie2 signaling pathway in the antiangiogenic action of triptolide. The discovery of an alternative pathway further confirms the value of ethnopharmacological investigations into traditional botanicals for leads for potential drug development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping