PUBLICATION
Identification of cis-acting elements repressing blue opsin expression in zebrafish UV cones and pineal cells
- Authors
- Takechi, M., Seno, S., and Kawamura, S.
- ID
- ZDB-PUB-080922-8
- Date
- 2008
- Source
- The Journal of biological chemistry 283(46): 31625-31632 (Journal)
- Registered Authors
- Kawamura, Shoji
- Keywords
- none
- MeSH Terms
-
- Animals
- Color
- Gene Expression Regulation*
- Genome/genetics
- Mutation/genetics
- Opsins/genetics
- Opsins/metabolism*
- Pineal Gland/cytology*
- Pineal Gland/metabolism
- Response Elements/genetics*
- Retina/metabolism
- Retinal Cone Photoreceptor Cells/metabolism*
- Ultraviolet Rays*
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 18796431 Full text @ J. Biol. Chem.
Citation
Takechi, M., Seno, S., and Kawamura, S. (2008) Identification of cis-acting elements repressing blue opsin expression in zebrafish UV cones and pineal cells. The Journal of biological chemistry. 283(46):31625-31632.
Abstract
Opsin genes are expressed in a cell-type specific manner in the retina and the pineal organ for visual and non-visual photoreceptive purposes, but the regulatory mechanism behind the tissue and cell selectivity is not well understood. In the present study, we focus on the expression regulation of the blue-sensitive opsin gene SWS2 of zebrafish by taking a transgenic approach using the green fluorescence protein (GFP) as an expression reporter. The zebrafish SWS2 is a single-copy gene and expressed specifically in the "long single cones" (LSCs) in the retina. We found that (1) a 0.3-kb region between 0.6 and 0.3 kb 5' of the SWS2 initiation codon, encompassing four cone-rod homeobox (Crx) binding sites (OTX sequences), contains the region necessary and sufficient to drive gene expression in LSCs, (2) a 15-bp portion (-341~-327) in the 0.3-kb region represses the gene expression in the "short single cones" (SSCs) which are dedicated to the UV-sensitive opsin gene SWS1, (3) an 11-bp sequence TAACTGCCAGT (-441~-431) in the 0.3-kb region, with its adjacent OTX element, also works as a repressor for gene expression in the pineal cells, and (4) this OTX site is necessary for expression repression in the bipolar cells in the retina. These findings open a way for understanding the complex interaction of positive and negative regulatory factors that governs the cell-type specificity of the opsin gene expression in the photoreceptive cells in the retina and the pineal organ. We termed the novel 11-bp sequence as the pineal negative regulatory element, PINE.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping