PUBLICATION
Transcriptional profiling of endogenous germ layer precursor cells identifies dusp4 as an essential gene in zebrafish endoderm specification
- Authors
- Brown, J.L., Snir, M., Noushmehr, H., Kirby, M., Hong, S.K., Elkahloun, A.G., and Feldman, B.
- ID
- ZDB-PUB-080826-43
- Date
- 2008
- Source
- Proceedings of the National Academy of Sciences of the United States of America 105(34): 12337-12342 (Journal)
- Registered Authors
- Feldman, Benjamin, Hong, Sung-Kook
- Keywords
- microarray, zgc:55423, gastrulation, mkp2, sox17
- Datasets
- GEO:GSE8654
- MeSH Terms
-
- High Mobility Group Proteins/deficiency*
- SOXF Transcription Factors
- Gene Expression Regulation, Developmental
- Embryonic Induction/genetics
- Zebrafish Proteins/deficiency*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/physiology
- Embryo, Nonmammalian
- Gene Expression Profiling
- Embryonic Development/genetics
- Transcription Factors/deficiency*
- Transcription, Genetic*
- Mitogen-Activated Protein Kinase Phosphatases/genetics*
- Mitogen-Activated Protein Kinase Phosphatases/physiology
- Germ Layers/cytology*
- Germ Layers/embryology
- DNA-Binding Proteins/deficiency*
- Animals
- Ectoderm/cytology
- Ectoderm/embryology
- Mesoderm/cytology
- Mesoderm/embryology
- Dual-Specificity Phosphatases/genetics*
- Dual-Specificity Phosphatases/physiology
- PubMed
- 18719100 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Brown, J.L., Snir, M., Noushmehr, H., Kirby, M., Hong, S.K., Elkahloun, A.G., and Feldman, B. (2008) Transcriptional profiling of endogenous germ layer precursor cells identifies dusp4 as an essential gene in zebrafish endoderm specification. Proceedings of the National Academy of Sciences of the United States of America. 105(34):12337-12342.
Abstract
A major goal for developmental biologists is to define the behaviors and molecular contents of differentiating cells. We have devised a strategy for isolating cells from diverse embryonic regions and stages in the zebrafish, using computer-guided laser photoconversion of injected Kaede protein and flow cytometry. This strategy enabled us to perform a genome-wide transcriptome comparison of germ layer precursor cells. Mesendoderm and ectoderm precursors cells isolated by this method differentiated appropriately in transplantation assays. Microarray analysis of these cells reidentified known genes at least as efficiently as previously reported strategies that relied on artificial mesendoderm activation or inhibition. We also identified a large set of uncharacterized mesendoderm-enriched genes as well as ectoderm-enriched genes. Loss-of-function studies revealed that one of these genes, the MAP kinase inhibitor dusp4, is essential for early development. Embryos injected with antisense morpholino oligonucleotides that targeted Dusp4 displayed necrosis of head tissues. Marker analysis during late gastrulation revealed a specific loss of sox17, but not of other endoderm markers, and analysis at later stages revealed a loss of foregut and pancreatic endoderm. This specific loss of sox17 establishes a new class of endoderm specification defect.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping