PUBLICATION
Endoderm-derived Sonic hedgehog and mesoderm Hand2 expression are required for enteric nervous system development in zebrafish
- Authors
- Reichenbach, B., Delalande, J.M., Kolmogorova, E., Prier, A., Nguyen, T., Smith, C.M., Holzschuh, J., and Shepherd, I.T.
- ID
- ZDB-PUB-080429-9
- Date
- 2008
- Source
- Developmental Biology 318(1): 52-64 (Journal)
- Registered Authors
- Holzschuh, Jochen, Shepherd, Iain T.
- Keywords
- Sonic hedgehog, Enteric nervous system, Neural crest, Zebrafish, Cell migration, GDNF, Hand2
- MeSH Terms
-
- Hedgehog Proteins/genetics
- Hedgehog Proteins/metabolism*
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/metabolism
- Endoderm/cytology
- Endoderm/metabolism*
- Enteric Nervous System/cytology
- Enteric Nervous System/embryology*
- Enteric Nervous System/metabolism
- Mesoderm/cytology
- Mesoderm/metabolism*
- In Situ Hybridization
- Cell Movement/physiology
- Animals
- Receptors, Cell Surface/genetics
- Receptors, Cell Surface/metabolism
- Signal Transduction/physiology
- Basic Helix-Loop-Helix Transcription Factors/genetics
- Basic Helix-Loop-Helix Transcription Factors/metabolism*
- Zebrafish*/anatomy & histology
- Zebrafish*/embryology
- Gene Expression Regulation, Developmental
- Stem Cells/cytology
- Stem Cells/physiology
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Veratrum Alkaloids/metabolism
- Animals, Genetically Modified
- High Mobility Group Proteins/genetics
- High Mobility Group Proteins/metabolism
- Glial Cell Line-Derived Neurotrophic Factor/genetics
- Glial Cell Line-Derived Neurotrophic Factor/metabolism
- SOX Transcription Factors
- Proto-Oncogene Proteins c-ret/genetics
- Proto-Oncogene Proteins c-ret/metabolism
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Body Patterning
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism
- PubMed
- 18436202 Full text @ Dev. Biol.
Citation
Reichenbach, B., Delalande, J.M., Kolmogorova, E., Prier, A., Nguyen, T., Smith, C.M., Holzschuh, J., and Shepherd, I.T. (2008) Endoderm-derived Sonic hedgehog and mesoderm Hand2 expression are required for enteric nervous system development in zebrafish. Developmental Biology. 318(1):52-64.
Abstract
The zebrafish enteric nervous system (ENS), like those of all other vertebrate species, is principally derived from the vagal neural crest cells (NCC). The developmental controls that govern the migration, proliferation and patterning of the ENS precursors are not well understood. We have investigated the roles of endoderm and Sonic hedgehog (SHH) in the development of the ENS. We show that endoderm is required for the migration of ENS NCC from the vagal region to the anterior end of the intestine. We show that the expression of shh and its receptor ptc-1 correlate with the development of the ENS and demonstrate that hedgehog (HH) signaling is required in two phases, a pre-enteric and an enteric phase, for normal ENS development. We show that HH signaling regulates the proliferation of vagal NCC and ENS precursors in vivo. We also show the zebrafish hand2 is required for the normal development of the intestinal smooth muscle and the ENS. Furthermore we show that endoderm and HH signaling, but not hand2, regulate gdnf expression in the intestine, highlighting a central role of endoderm and SHH in patterning the intestine and the ENS.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping