PUBLICATION
Foxn4 directly regulates tbx2b expression and atrioventricular canal formation
- Authors
- Chi, N.C., Shaw, R.M., De Val, S., Kang, G., Jan, L.Y., Black, B.L., and Stainier, D.Y.
- ID
- ZDB-PUB-080326-29
- Date
- 2008
- Source
- Genes & Development 22(6): 734-739 (Journal)
- Registered Authors
- Chi, Neil C., Stainier, Didier
- Keywords
- Atrioventricular canal, evolutionary development, Forkhead transcription factors, T-box transcription factors, calcium indicator, mutations
- MeSH Terms
-
- Heart Ventricles/embryology*
- Heart Ventricles/metabolism
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Forkhead Transcription Factors/physiology*
- Electrophoretic Mobility Shift Assay
- Heart Atria/embryology*
- Heart Atria/metabolism
- Chromosome Mapping
- Immunoenzyme Techniques
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/physiology
- Zebrafish
- Animals
- Gene Expression Regulation, Developmental
- DNA Primers
- Animals, Genetically Modified
- Binding Sites
- Atrioventricular Node/embryology*
- Zebrafish Proteins/physiology*
- T-Box Domain Proteins/physiology*
- PubMed
- 18347092 Full text @ Genes & Dev.
Citation
Chi, N.C., Shaw, R.M., De Val, S., Kang, G., Jan, L.Y., Black, B.L., and Stainier, D.Y. (2008) Foxn4 directly regulates tbx2b expression and atrioventricular canal formation. Genes & Development. 22(6):734-739.
Abstract
Cardiac chamber formation represents an essential evolutionary milestone that allows for the heart to receive (atrium) and pump (ventricle) blood throughout a closed circulatory system. Here, we reveal a novel transcriptional pathway between foxn4 and tbx genes that facilitates this evolutionary event. We show that the zebrafish gene slipjig, which encodes Foxn4, regulates the formation of the atrioventricular (AV) canal to divide the heart. sli/foxn4 is expressed in the AV canal, and its encoded product binds to a highly conserved tbx2 enhancer domain that contains Foxn4- and T-box-binding sites, both necessary to regulate tbx2b expression in the AV canal.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping