PUBLICATION
Multiple roles of the furrow deepening Ca2+ transient during cytokinesis in zebrafish embryos
- Authors
- Li, W.M., Webb, S.E., Chan, C.M., and Miller, A.L.
- ID
- ZDB-PUB-080306-37
- Date
- 2008
- Source
- Developmental Biology 316(2): 228-248 (Journal)
- Registered Authors
- Miller, Andrew L., Webb, Sarah E.
- Keywords
- none
- MeSH Terms
-
- Actins/metabolism
- Animals
- Calcium/physiology*
- Cytokinesis/physiology*
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/physiology*
- Embryonic Development
- Genes, Reporter
- Immunohistochemistry
- Kinetics
- Plasmids
- Recombinant Fusion Proteins/metabolism
- Zebrafish/embryology*
- PubMed
- 18313658 Full text @ Dev. Biol.
Citation
Li, W.M., Webb, S.E., Chan, C.M., and Miller, A.L. (2008) Multiple roles of the furrow deepening Ca2+ transient during cytokinesis in zebrafish embryos. Developmental Biology. 316(2):228-248.
Abstract
The generation of a required series of localized Ca(2+) transients during cytokinesis in zebrafish embryos suggests that Ca(2+) plays a necessary role in regulating this process. Here, we report that cortical actin remodeling, characterized by the reorganization of the contractile band and the formation during furrow deepening of pericleavage F-actin enrichments (PAEs), requires a localized increase in intracellular Ca(2+), which is released from IP(3)-sensitive stores. We demonstrate that VAMP-2 vesicle fusion at the deepening furrow also requires Ca(2+) released via IP(3) receptors, as well as the presence of PAEs and the action of calpains. Finally, by expressing a dominant-negative form of the kinesin-like protein, kif23, we demonstrate that its recruitment to the furrow region is required for VAMP-2 vesicle transport; and via FRAP analysis, that kif23 localization is also Ca(2+)-dependent. Collectively, our data demonstrate that a localized increase in intracellular Ca(2+) is involved in regulating several key events during furrow deepening and subsequent apposition.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping