PUBLICATION
Molecular multi-effect screening of environmental pollutants using the MolDarT
- Authors
- Liedtke, A., Muncke, J., Rüfenacht, K., and Eggen, R.I.
- ID
- ZDB-PUB-080218-27
- Date
- 2008
- Source
- Environmental toxicology 23(1): 59-67 (Journal)
- Registered Authors
- Keywords
- developing zebrafish, immune disruption, detoxification, metal toxicity, molecular effect, rag1, metallothionein 2, cyp1a1
- MeSH Terms
-
- Animals
- Cadmium Chloride/toxicity
- Cytochrome P-450 CYP1A1/drug effects
- Cytochrome P-450 CYP1A1/genetics
- DNA Primers
- Environmental Exposure*
- Environmental Monitoring/methods
- Environmental Pollutants/toxicity*
- Estrogens/toxicity*
- Gene Expression/drug effects
- Naphthalenes/toxicity
- RNA, Messenger/analysis
- Reverse Transcriptase Polymerase Chain Reaction
- Zebrafish/genetics*
- Zebrafish Proteins/drug effects*
- Zebrafish Proteins/genetics
- Zinc Sulfate/toxicity
- PubMed
- 18214934 Full text @ Env. Tox.
- CTD
- 18214934
Citation
Liedtke, A., Muncke, J., Rüfenacht, K., and Eggen, R.I. (2008) Molecular multi-effect screening of environmental pollutants using the MolDarT. Environmental toxicology. 23(1):59-67.
Abstract
Molecular effect detection is a useful approach for ecotoxicological screening of chemicals. We show here the application of the molecular DarT (MolDarT), where the expression of selected target genes is detected in short-term (120 h) exposed developing zebrafish (Danio rerio), thus allowing subacute multi-effect compound screening. The genes metallothionein 2 (mt2), cytochrome P450 1A1 (cyp1a1), and recombination activation gene 1 (rag1) are used as endpoints that describe detoxification/metal toxicity (mt2), detoxification/PAH toxicity (cyp1a1), and acquired immune system disruption (rag1). Each gene's developmental expression was studied in unexposed zebrafish during 4 to 120 h past fertilization (hpf), and all three genes were found to be expressed at 120 hpf. Furthermore, mt2 transcripts were present at high levels at 4 hpf, indicating a maternal transfer. For positive toxicity controls, freshly fertilized zebrafish eggs were exposed for 120 hpf to ZnSO(4), 1,5-dimethylnaphthalene (DMN) and CdCl(2). Exposure to 100 and 200 muM ZnSO(4) significantly induced mt2; 10 muM DMN and 20 muM DMN resulted in significantly increased cyp1a1 abundance; and 5 and 10 muM CdCl(2) significantly reduced rag1 expression levels. Furthermore, we analysed these target genes for their expression in zebrafish eggs from a previous exposure study. The eggs were exposed for 120 hpf to the environmental pollutants estradiol (E2), ethinylestradiol (EE2), nonylphenol (NP), atrazine, cyproconazol, and bisphenol A (BPA) and found differential expression of the three genes. Exposure to the (xeno-)estrogenic compound NP (0.75 muM) significantly lowered mt2 expression. This study shows the potential of short-term in vivo multi-effect screenings within one single subacute exposure using the MolDarT.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping