PUBLICATION
Heat-shock protein 90alpha1 is required for organized myofibril assembly in skeletal muscles of zebrafish embryos
- Authors
- Du, S.J., Li, H., Bian, Y., and Zhong, Y.
- ID
- ZDB-PUB-080209-1
- Date
- 2008
- Source
- Proceedings of the National Academy of Sciences of the United States of America 105(2): 554-559 (Journal)
- Registered Authors
- Du, Shao Jun (Jim)
- Keywords
- myofibrillogenesis, unc45, myosin chaperone, Hsp90
- MeSH Terms
-
- Amino Acid Sequence
- Zebrafish
- Molecular Sequence Data
- Muscle Proteins/chemistry
- Myosins/chemistry
- Myofibrils/metabolism*
- Muscle, Skeletal/embryology*
- Gene Expression Regulation, Developmental*
- Oligonucleotides, Antisense/chemistry
- Molecular Chaperones/metabolism
- HSP90 Heat-Shock Proteins/chemistry*
- Sequence Homology, Amino Acid
- Animals
- Base Sequence
- Zebrafish Proteins/chemistry
- Sequence Homology, Nucleic Acid
- PubMed
- 18182494 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Du, S.J., Li, H., Bian, Y., and Zhong, Y. (2008) Heat-shock protein 90alpha1 is required for organized myofibril assembly in skeletal muscles of zebrafish embryos. Proceedings of the National Academy of Sciences of the United States of America. 105(2):554-559.
Abstract
Heat-shock protein 90alpha (Hsp90alpha) is a member of the molecular chaperone family involved in protein folding and assembly. The role of Hsp90alpha in the developmental process, however, remains unclear. Here we report that zebrafish contains two Hsp90alpha genes, Hsp90alpha1, and Hsp90alpha2. Hsp90alpha1 is specifically expressed in developing somites and skeletal muscles of zebrafish embryos. We have demonstrated that Hsp90alpha1 is essential for myofibril organization in skeletal muscles of zebrafish embryos. Knockdown of Hsp90alpha1 resulted in paralyzed zebrafish embryos with poorly organized myofibrils in skeletal muscles. In contrast, knockdown of Hsp90alpha2 had no effect on muscle contraction and myofibril organization. The filament defects could be rescued in a cell autonomous manner by an ectopic expression of Hsp90alpha1. Biochemical analyses revealed that knockdown of Hsp90alpha1 resulted in significant myosin degradation and up-regulation of unc-45b gene expression. These results indicate that Hsp90alpha1 plays an important role in muscle development, likely through facilitating myosin folding and assembly into organized myofibril filaments.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping