PUBLICATION
Ca2+ channel-independent requirement for MAGUK family CACNB4 genes in initiation of zebrafish epiboly
- Authors
- Ebert, A.M., McAnelly, C.A., Srinivasan, A., Linker, J.L., Horne, W.A., and Garrity, D.M.
- ID
- ZDB-PUB-080124-6
- Date
- 2008
- Source
- Proceedings of the National Academy of Sciences of the United States of America 105(1): 198-203 (Journal)
- Registered Authors
- Garrity, Deborah, Srinivasan, Ashok
- Keywords
- β4 subunit, yolk syncytial layer, nocodazole
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Calcium Channels/biosynthesis
- Calcium Channels/genetics
- Calcium Channels/metabolism*
- Calcium Channels, L-Type/metabolism*
- Electrophysiology
- Gene Expression Regulation*
- Humans
- Microscopy, Confocal
- Molecular Sequence Data
- Nocodazole/pharmacology
- Protein Structure, Tertiary
- Sequence Homology, Amino Acid
- Tubulin Modulators/pharmacology
- Xenopus laevis
- Zebrafish
- Zebrafish Proteins/biosynthesis
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 18172207 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Ebert, A.M., McAnelly, C.A., Srinivasan, A., Linker, J.L., Horne, W.A., and Garrity, D.M. (2008) Ca2+ channel-independent requirement for MAGUK family CACNB4 genes in initiation of zebrafish epiboly. Proceedings of the National Academy of Sciences of the United States of America. 105(1):198-203.
Abstract
CACNB genes encode membrane-associated guanylate kinase (MAGUK) proteins once thought to function exclusively as auxiliary beta subunits in assembly and gating of voltage-gated Ca(2+) channels. Here, we report that zygotic deficiency of zebrafish beta4 protein blocks initiation of epiboly, the first morphogenetic movement of teleost embryos. Reduced beta4 function in the yolk syncytial layer (YSL) leads to abnormal division and dispersal of yolk syncytial nuclei, blastoderm retraction, and death, effects highly similar to microtubule disruption by nocodazole. Epiboly is restored by coinjection of human beta4 cRNA or, surprisingly, by mutant cRNA encoding beta4 subunits incapable of binding to Ca(2+) channel alpha1 subunits. This study defines a YSL-driven zygotic mechanism essential for epiboly initiation and reveals a Ca(2+) channel-independent beta4 protein function potentially involving the cytoskeleton.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping