PUBLICATION

Emilin genes are duplicated and dynamically expressed during zebrafish embryonic development

Authors
Milanetto, M., Tiso, N., Braghetta, P., Volpin, D., Argenton, F., and Bonaldo, P.
ID
ZDB-PUB-071227-11
Date
2008
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   237(1): 222-232 (Journal)
Registered Authors
Argenton, Francesco, Milanetto, Martina, Tiso, Natascia
Keywords
extracellular matrix, emilins, zebrafish, embryonic development, gene expression, in situ hybridization
MeSH Terms
  • Animals
  • Computational Biology
  • Databases, Genetic
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism*
  • Gene Expression Regulation, Developmental*
  • In Situ Hybridization
  • Membrane Glycoproteins/genetics*
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
PubMed
18095348 Full text @ Dev. Dyn.
Abstract
Emilins are a family of extracellular matrix proteins with common structural organization and containing a characteristic N-terminal cysteine-rich domain. The prototype of this family, Emilin-1, is found in human and murine organs in association with elastic fibers, and other emilins were recently isolated in mammals. To gain insight into these proteins in lower vertebrates, we investigated the expression of emilins in the fish Danio rerio. Using sequence similarity tools, we identified eight members of this family in zebrafish. Each emilin gene has two paralogs in zebrafish, showing conserved structure with the human ortholog. In situ hybridization revealed that expression of zebrafish emilin genes is regulated in a spatiotemporal manner during embryonic development, with overlapping and site-specific patterns mostly including mesenchymal structures. Expression of certain emilin genes in peculiar areas, such as the central nervous system or the posterior notochord, suggests that they may play a role in key morphogenetic processes.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping