PUBLICATION
Consequences of Hoxb1 duplication in teleost fish
- Authors
- Hurley, I.A., Scemama, J.L., and Prince, V.E.
- ID
- ZDB-PUB-071118-1
- Date
- 2007
- Source
- Evolution & development 9(6): 540-554 (Journal)
- Registered Authors
- Hurley, Imogen, Prince, Victoria E.
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Fishes/genetics*
- Gene Duplication
- Homeodomain Proteins/genetics*
- Molecular Sequence Data
- Sequence Alignment
- Sequence Analysis, DNA
- PubMed
- 17976051 Full text @ Evol. Dev.
Citation
Hurley, I.A., Scemama, J.L., and Prince, V.E. (2007) Consequences of Hoxb1 duplication in teleost fish. Evolution & development. 9(6):540-554.
Abstract
Vertebrate evolution is characterized by gene and genome duplication events. There is strong evidence that a whole-genome duplication occurred in the lineage leading to the teleost fishes. We have focused on the teleost hoxb1 duplicate genes as a paradigm to investigate the consequences of gene duplication. Previous analysis of the duplicated zebrafish hoxb1 genes suggested they have subfunctionalized. The combined expression pattern of the two zebrafish hoxb1 genes recapitulates the expression pattern of the single Hoxb1 gene of tetrapods, possibly due to degenerative changes in complementary cis-regulatory elements of the duplicates. Here we have tested the hypothesis that all teleost duplicates had a similar fate post duplication, by examining hoxb1 genes in medaka and striped bass. Consistent with this theory, we found that the ancestral Hoxb1 expression pattern is subdivided between duplicate genes in a largely similar fashion in zebrafish, medaka, and striped bass. Further, our analysis of hoxb1 genes reveals that sequence changes in cis-regulatory regions may underlie subfunctionalization in all teleosts, although the specific changes vary between species. It was previously shown that zebrafish hoxb1 duplicates have also evolved different functional capacities. We used misexpression to compare the functions of hoxb1 duplicates from zebrafish, medaka and striped bass. Unexpectedly, we found that some biochemical properties, which were paralog specific in zebrafish, are conserved in both duplicates of other species. This work suggests that the fate of duplicate genes varies across the teleost group.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping