PUBLICATION
Role of Bone morphogenetic protein 4 in zebrafish semicircular canal development
- Authors
- Omata, Y., Nojima, Y., Nakayama, S., Okamoto, H., Nakamura, H., and Funahashi, J.I.
- ID
- ZDB-PUB-071009-7
- Date
- 2007
- Source
- Development, growth & differentiation 49(9): 711-719 (Journal)
- Registered Authors
- Nakayama, Satomi, Okamoto, Hitoshi
- Keywords
- none
- MeSH Terms
-
- Actins/metabolism
- Animals
- Body Patterning/drug effects
- Bone Morphogenetic Protein 2
- Bone Morphogenetic Protein 4
- Bone Morphogenetic Proteins/genetics
- Bone Morphogenetic Proteins/metabolism
- Bone Morphogenetic Proteins/physiology*
- Carrier Proteins/pharmacology
- Ear, Inner/drug effects
- Ear, Inner/embryology
- Ear, Inner/metabolism
- Gene Expression Regulation, Developmental/drug effects
- Humans
- Immunohistochemistry
- In Situ Hybridization
- Mice
- Microscopy, Confocal
- Phenotype
- Recombinant Proteins/metabolism
- Recombinant Proteins/pharmacology
- Semicircular Canals/drug effects
- Semicircular Canals/embryology*
- Semicircular Canals/metabolism
- Transforming Growth Factor beta/genetics
- Transforming Growth Factor beta/metabolism
- Transforming Growth Factor beta/physiology
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish Proteins/physiology*
- PubMed
- 17908182 Full text @ Dev. Growth Diff.
Citation
Omata, Y., Nojima, Y., Nakayama, S., Okamoto, H., Nakamura, H., and Funahashi, J.I. (2007) Role of Bone morphogenetic protein 4 in zebrafish semicircular canal development. Development, growth & differentiation. 49(9):711-719.
Abstract
Bone morphogenetic proteins (BMPs) are known to play roles in inner ear development of higher vertebrates. In zebrafish, there are several reports showing that members of the BMP family are expressed in the otic vesicle. We have isolated a novel zebrafish mutant gallery, which affects the development of the semicircular canal. Gallery merely forms the lateral and the immature anterior protrusion, and does not form posterior and ventral protrusions. We found that the expression of bmp2b and bmp4, both expressed in the normal optic vesicle at the protrusion stage, are extremely upregulated in the otic vesicle of gallery. To elucidate the role of BMPs in the development of the inner ear of zebrafish, we have applied excess BMP to the wild-type otic vesicle. The formation of protrusions was severely affected, and in some cases, they were completely lost in BMP4-treated embryos. Furthermore, the protrusions in gallery treated with Noggin were partially rescued. These data indicate that BMP4 plays an important role in the development of protrusions to form semicircular canals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping