PUBLICATION
Identification and characterization of zebrafish semaphorin 6D
- Authors
- Kimura, M., Taniguchi, M., Mikami, Y., Masuda, T., Yoshida, T., Mishina, M., and Shimizu, T.
- ID
- ZDB-PUB-071001-12
- Date
- 2007
- Source
- Biochemical and Biophysical Research Communications 363(3): 762-768 (Journal)
- Registered Authors
- Mikami, Yoshinori, Mishina, Masayoshi, Yoshida, Tomoyuki
- Keywords
- Semaphorin, Plexin, Axon guidance, Brain, Lens, Rhombomere, Repellent
- MeSH Terms
-
- Animals
- Cell Line
- Chick Embryo
- Cloning, Molecular
- DNA, Complementary/chemistry
- DNA, Complementary/genetics
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Ganglia, Spinal/embryology
- Ganglia, Spinal/metabolism
- Gene Expression Profiling*
- Gene Expression Regulation, Developmental
- Humans
- In Situ Hybridization
- Molecular Sequence Data
- Protein Binding
- Protein Isoforms/genetics
- Protein Isoforms/metabolism
- Receptors, Cell Surface/genetics
- Receptors, Cell Surface/metabolism
- Semaphorins/genetics*
- Semaphorins/metabolism
- Sequence Analysis, DNA
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 17897628 Full text @ Biochem. Biophys. Res. Commun.
Citation
Kimura, M., Taniguchi, M., Mikami, Y., Masuda, T., Yoshida, T., Mishina, M., and Shimizu, T. (2007) Identification and characterization of zebrafish semaphorin 6D. Biochemical and Biophysical Research Communications. 363(3):762-768.
Abstract
The semaphorin gene family contains a large number of secreted and transmembrane proteins; some function as repulsive and attractive cues of axon guidance during development. Here, we report cloning and characterization of zebrafish transmembrane semaphorin gene, semaphorin 6D (sema6D). Sema6D is expressed predominantly in the nervous system during embryogenesis, as determined by in situ hybridization. We also found that Sema6D binds Plexin-A1 in vitro, but not other Plexins. It induces the repulsion of dorsal root ganglion axons, but not sympathetic axons. Consequently, Sema6D might use Plexin-A1 as a receptor to repel specific types of axons during development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping