PUBLICATION
Generation of a transgenic zebrafish model of Tauopathy using a novel promoter element derived from the zebrafish eno2 gene
- Authors
- Bai, Q., Garver, J.A., Hukriede, N.A., and Burton, E.A.
- ID
- ZDB-PUB-071001-11
- Date
- 2007
- Source
- Nucleic acids research 35(19): 6501-6516 (Journal)
- Registered Authors
- Burton, Edward A., Hukriede, Neil
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Animals, Genetically Modified/genetics
- Animals, Genetically Modified/metabolism
- Brain/metabolism
- Disease Models, Animal*
- Molecular Sequence Data
- Phosphopyruvate Hydratase/genetics*
- Phosphopyruvate Hydratase/metabolism
- Promoter Regions, Genetic*
- Repetitive Sequences, Amino Acid
- Sequence Analysis, DNA
- Tauopathies/genetics*
- Transcription Initiation Site
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- tau Proteins/chemistry
- tau Proteins/genetics*
- tau Proteins/metabolism
- PubMed
- 17897967 Full text @ Nucleic Acids Res.
Citation
Bai, Q., Garver, J.A., Hukriede, N.A., and Burton, E.A. (2007) Generation of a transgenic zebrafish model of Tauopathy using a novel promoter element derived from the zebrafish eno2 gene. Nucleic acids research. 35(19):6501-6516.
Abstract
The aim of this study was to isolate cis-acting regulatory elements for the generation of transgenic zebrafish models of neurodegeneration. Zebrafish enolase-2 (eno2) showed neuronal expression increasing from 24 to 72 h post-fertilization (hpf) and persisting through adulthood. A 12 kb eno2 genomic fragment, extending from 8 kb upstream of exon 1 to exon 2, encompassing intron 1, was sufficient to drive neuronal reporter gene expression in vivo over a similar time course. Five independent lines of stable Tg(eno2 : GFP) zebrafish expressed GFP widely in neurons, including populations with relevance to neurodegeneration, such as cholinergic neurons, dopaminergic neurons and cerebellar Purkinje cells. We replaced the exon 2-GFP fusion gene with a cDNA encoding the 4-repeat isoform of the human microtubule-associated protein Tau. The first intron of eno2 was spliced with high fidelity and efficiency from the chimeric eno2-Tau transcript. Tau was expressed at approximately 8-fold higher levels in Tg(eno2 : Tau) zebrafish brain than normal human brain, and localized to axons, neuropil and ectopic neuronal somatic accumulations resembling neurofibrillary tangles. The 12 kb eno2 promoter drives high-level transgene expression in differentiated neurons throughout the CNS of stable transgenic zebrafish. This regulatory element will be useful for the construction of transgenic zebrafish models of neurodegeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping