PUBLICATION
Remote control of neuronal activity with a light-gated glutamate receptor
- Authors
- Szobota, S., Gorostiza, P., Del Bene, F., Wyart, C., Fortin, D.L., Kolstad, K.D., Tulyathan, O., Volgraf, M., Numano, R., Aaron, H.L., Scott, E.K., Kramer, R.H., Flannery, J., Baier, H., Trauner, D., and Isacoff, E.Y.
- ID
- ZDB-PUB-070614-5
- Date
- 2007
- Source
- Neuron 54(4): 535-545 (Journal)
- Registered Authors
- Baier, Herwig, Del Bene, Filippo, Isacoff, Ehud, Scott, Ethan, Wyart, Claire
- Keywords
- none
- MeSH Terms
-
- Hippocampus/cytology
- Larva
- Mutation
- Patch-Clamp Techniques/methods
- Neurons/drug effects
- Neurons/physiology*
- Neurons/radiation effects
- Behavior, Animal/physiology*
- Behavior, Animal/radiation effects
- Zebrafish
- Lighting/methods*
- Receptors, Kainic Acid/genetics
- Receptors, Kainic Acid/physiology*
- Animals, Newborn
- Rats
- Transfection/methods
- Physical Stimulation/methods
- Cysteine/genetics
- Cells, Cultured
- Dose-Response Relationship, Radiation
- Leucine/genetics
- Action Potentials/physiology
- Action Potentials/radiation effects
- Animals, Genetically Modified
- Electric Stimulation/methods
- Animals
- Excitatory Postsynaptic Potentials
- PubMed
- 17521567 Full text @ Neuron
Citation
Szobota, S., Gorostiza, P., Del Bene, F., Wyart, C., Fortin, D.L., Kolstad, K.D., Tulyathan, O., Volgraf, M., Numano, R., Aaron, H.L., Scott, E.K., Kramer, R.H., Flannery, J., Baier, H., Trauner, D., and Isacoff, E.Y. (2007) Remote control of neuronal activity with a light-gated glutamate receptor. Neuron. 54(4):535-545.
Abstract
The ability to stimulate select neurons in isolated tissue and in living animals is important for investigating their role in circuits and behavior. We show that the engineered light-gated ionotropic glutamate receptor (LiGluR), when introduced into neurons, enables remote control of their activity. Trains of action potentials are optimally evoked and extinguished by 380 nm and 500 nm light, respectively, while intermediate wavelengths provide graded control over the amplitude of depolarization. Light pulses of 1-5 ms in duration at approximately 380 nm trigger precisely timed action potentials and EPSP-like responses or can evoke sustained depolarizations that persist for minutes in the dark until extinguished by a short pulse of approximately 500 nm light. When introduced into sensory neurons in zebrafish larvae, activation of LiGluR reversibly blocks the escape response to touch. Our studies show that LiGluR provides robust control over neuronal activity, enabling the dissection and manipulation of neural circuitry in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping