PUBLICATION
A Large-scale Mutagenesis Screen to Identify Seizure-resistant Zebrafish
- Authors
- Baraban, S.C., Dinday, M.T., Castro, P.A., Chege, S., Guyenet, S., and Taylor, M.R.
- ID
- ZDB-PUB-070614-4
- Date
- 2007
- Source
- Epilepsia 48(6): 1151-1157 (Journal)
- Registered Authors
- Taylor, Michael
- Keywords
- none
- MeSH Terms
-
- Animals
- Behavior, Animal/drug effects
- Behavior, Animal/physiology
- Disease Models, Animal*
- Genetic Predisposition to Disease/genetics
- Genetic Testing*
- Larva/genetics
- Larva/physiology
- Locomotion/drug effects
- Locomotion/genetics
- Mutagenesis/genetics*
- Pentylenetetrazole/pharmacology
- Seizures/chemically induced
- Seizures/epidemiology
- Seizures/prevention & control*
- Status Epilepticus/chemically induced
- Status Epilepticus/prevention & control
- Superior Colliculi/physiology
- Zebrafish/genetics*
- PubMed
- 17521353 Full text @ Epilepsia
Citation
Baraban, S.C., Dinday, M.T., Castro, P.A., Chege, S., Guyenet, S., and Taylor, M.R. (2007) A Large-scale Mutagenesis Screen to Identify Seizure-resistant Zebrafish. Epilepsia. 48(6):1151-1157.
Abstract
Methods: Seizures were induced with pentylenetetrazole (PTZ). Zebrafish were analyzed between 3 and 7 days postfertilization (dpf). Genome mutations were induced in founders by using N-ethyl-nitrosourea (ENU). Seizure behavior was monitored by using a high-speed camera and quantified by locomotion-tracking software. Electrographic activity was monitored by using a field-recording electrode placed in the optic tectum of agar-immobilized zebrafish. Results: Short-term PTZ exposure elicited a burst-suppression seizure pattern in 3-dpf zebrafish and more complex activity consisting of interictal- and ictal-like discharges at 7 dpf. Prolonged exposure to PTZ induced status epilepticus-like seizure activity and fatality in wild-type zebrafish larvae. With a PTZ survival assay at 6-7 dpf, we identified six zebrafish mutants in a forward-genetic screen covering nearly 2,000 F(2) families. One mutant (s334) also was shown to exhibit reduced behavioral activity on short-term PTZ exposure and an inability to generate long-duration ictal-like discharge. Conclusions: Zebrafish offers a powerful tool for the identification and study of a genetic basis for seizure resistance.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping