PUBLICATION
Spatiotemporal expression of the creatine metabolism related genes agat, gamt and ct1 during zebrafish embryogenesis
- Authors
- Wang, L., Zhang, Y., Shao, M., and Zhang, H.
- ID
- ZDB-PUB-070513-20
- Date
- 2007
- Source
- The International journal of developmental biology 51(3): 247-253 (Journal)
- Registered Authors
- Zhang, Ying
- Keywords
- agat, gamt, ct1, zebrafish, developmental expression
- MeSH Terms
-
- Amidinotransferases/genetics
- Amidinotransferases/metabolism*
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- Consensus Sequence
- Creatine/genetics
- Creatine/metabolism*
- DNA, Complementary
- Embryo, Nonmammalian
- Embryonic Development*
- Gene Expression Regulation, Developmental
- Guanidinoacetate N-Methyltransferase/genetics
- Guanidinoacetate N-Methyltransferase/metabolism*
- In Situ Hybridization
- Membrane Transport Proteins/genetics
- Membrane Transport Proteins/metabolism*
- Molecular Sequence Data
- Phylogeny
- Sequence Homology, Amino Acid
- Sequence Homology, Nucleic Acid
- Time Factors
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 17486546 Full text @ Int. J. Dev. Biol.
Citation
Wang, L., Zhang, Y., Shao, M., and Zhang, H. (2007) Spatiotemporal expression of the creatine metabolism related genes agat, gamt and ct1 during zebrafish embryogenesis. The International journal of developmental biology. 51(3):247-253.
Abstract
Glycine amidinotransferase (AGAT or GATM), guanidinoacetate methyltransferase (GAMT) and creatine transporter (CT1) are three proteins involved in the synthesis and uptake of creatine. The expression patterns of these three genes were examined in zebrafish embryos by whole mount in situ hybridization followed by histological sectioning. Expression of agat first appeared in the yolk syncytial layer (YSL) at the gastrula stage and was progressively up regulated during gastrulation. As development proceeds, agat was expressed in the mature somites during the segmentation stage and in the liver at 48 hpf. gamt showed a similar expression pattern to that of agat during embryogenesis. It was first detected in the center of the yolk from the cleavage to the gastrula stage. At the bud stage, its expression shifted to the YSL. gamt was also transiently expressed in the mature somites from 16 hpf to 24 hpf and became strongly expressed in the liver and in epithelial cells of the gut at 48 hpf. ct1 was initially uniformly expressed from the cleavage to the early segmentation stage; it was then strongly expressed in all the somites till 30 hpf and in the gut of 48 hpf embryos. However, ct1 transcripts also appeared in the central nervous system during the segmentation stage, but not in the YSL, the yolk or the liver. Our data reveal for the first time distinct and unique patterns of expression of the creatine metabolism genes agat, gamt and ct1 during zebrafish embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping