PUBLICATION
Targeted gene expression in the zebrafish prechordal plate
- Authors
- Inbal, A., Topczewski, J., and Solnica-Krezel, L.
- ID
- ZDB-PUB-061227-10
- Date
- 2006
- Source
- Genesis (New York, N.Y. : 2000) 44(12): 584-588 (Journal)
- Registered Authors
- Inbal, Adi, Solnica-Krezel, Lilianna, Topczewski, Jacek
- Keywords
- Gal4-VP16, UAS, goosecoid, GFP
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Crosses, Genetic
- Endoderm/metabolism*
- Gene Expression*
- In Situ Hybridization
- Nervous System/embryology*
- Nervous System/metabolism
- Trans-Activators/genetics*
- Zebrafish/embryology*
- Zebrafish/metabolism
- PubMed
- 17133414 Full text @ Genesis
Citation
Inbal, A., Topczewski, J., and Solnica-Krezel, L. (2006) Targeted gene expression in the zebrafish prechordal plate. Genesis (New York, N.Y. : 2000). 44(12):584-588.
Abstract
Targeted gene expression is a powerful tool for understanding gene function in vivo. In zebrafish, overexpression of gene products is typically accomplished ubiquitously, without temporal and spatial specificity. However, the yeast Gal4/UAS system can be used for targeted gene expression in zebrafish. Here we describe the generation and characterization of Tg[gsc: Gal4-VP16] transgenic zebrafish lines that harbor a construct encoding Gal4-VP16 transcriptional activator under the control of a fragment of the goosecoid gene promoter. Tg[gsc:Gal4-VP16] embryos express Gal4-VP16 RNA in presumptive prechordal plate mesendoderm during late blastula and throughout gastrulation. By crossing these fish to Tg[UAS-GFP] transgenic fish, we show that the gsc:Gal4-VP16 transgene is capable of driving strong expression of a target gene in the prechordal plate and its derivatives during gastrulation and segmentation. Thus, the use of Tg[gsc:Gal4-VP16] fish can help in understanding gene function in the prechordal plate, an embryonic structure that is crucial for normal neural patterning.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping