PUBLICATION

Evolution of teleostean hatching enzyme genes and their paralogous genes

Authors
Kawaguchi, M., Yasumasu, S., Hiroi, J., Naruse, K., Inoue, M., and Iuchi, I.
ID
ZDB-PUB-061020-16
Date
2006
Source
Development genes and evolution   216(12): 769-784 (Journal)
Registered Authors
Naruse, Kiyoshi
Keywords
Hatching enzyme, Astacin protease family, Intron-loss evolution, Medaka
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Chromosomes
  • Cloning, Molecular
  • Consensus Sequence
  • Conserved Sequence
  • DNA, Complementary/genetics
  • Databases, Genetic
  • Embryo, Nonmammalian
  • Evolution, Molecular*
  • Exons
  • Gene Expression
  • In Situ Hybridization
  • Introns
  • Metalloendopeptidases/chemistry
  • Metalloendopeptidases/genetics*
  • Metalloendopeptidases/metabolism
  • Molecular Sequence Data
  • Multigene Family/genetics
  • Nucleic Acid Amplification Techniques
  • Oryzias/embryology*
  • Oryzias/genetics*
  • Oryzias/metabolism
  • Phylogeny
  • Sequence Homology, Amino Acid
  • Synteny
PubMed
17016731 Full text @ Dev. Genes Evol.
Abstract
We isolated genes for hatching enzymes and their paralogs having two cysteine residues at their N-terminal regions in addition to four cysteines conserved in all the astacin family proteases. Genes for such six-cysteine-containing astacin proteases (C6AST) were searched out in the medaka genome database. Five genes for MC6AST1 to 5 were found in addition to embryo-specific hatching enzyme genes. RT-PCR and whole-mount in situ hybridization evidenced that MC6AST1 was expressed in embryos and epidermis of almost all adult tissues examined, while MC6AST2 and 3 were in mesenterium, intestine, and testis. MC6AST4 and 5 were specifically expressed in jaw. In addition, we cloned C6AST cDNA homologs from zebrafish, ayu, and fugu. The MC6AST1 to 5 genes were classified into three groups in the phylogenetic positions, and the expression patterns and hatching enzymes were clearly discriminated from other C6ASTs. Analysis of the exon-intron structures clarified that genes for hatching enzymes MHCE and MAHCE were intron-less, while other MC6AST genes were basically the same as the gene for another hatching enzyme MLCE. In the basal Teleost, the C6AST genes having the ancestral exon-intron structure (nine exon/eight intron structure) first appeared by duplication and chromosomal translocation. Thereafter, maintaining such ancestral exon-intron structure, the LCE gene was newly diversified in Euteleostei, and the MC6AST1 to 5 gene orthologs were duplicated and diversified independently in respective fish lineages. The HCE gene lost all introns in Euteleostei, whereas in the lineage to zebrafish, it was translocated from chromosome to chromosome and lost some of its introns.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping