PUBLICATION
Dual roles for adenomatous polyposis coli in regulating retinoic acid biosynthesis and Wnt during ocular development
- Authors
- Nadauld, L.D., Chidester, S., Shelton, D.N., Rai, K., Broadbent, T., Sandoval, I.T., Peterson, P.W., Manos, E.J., Ireland, C.M., Yost, H.J., and Jones, D.A.
- ID
- ZDB-PUB-060906-11
- Date
- 2006
- Source
- Proceedings of the National Academy of Sciences of the United States of America 103(36): 13409-13414 (Journal)
- Registered Authors
- Broadbent, Talmage, Yost, H. Joseph
- Keywords
- colon cancer, retina, APC
- MeSH Terms
-
- Adenomatous Polyposis Coli Protein/deficiency
- Adenomatous Polyposis Coli Protein/genetics
- Adenomatous Polyposis Coli Protein/metabolism*
- Animals
- Eye/drug effects
- Eye/embryology*
- Gene Expression Regulation, Developmental
- Homozygote
- Microinjections
- Molecular Sequence Data
- Mutation
- Oligonucleotides, Antisense/pharmacology
- Tretinoin/metabolism*
- Tretinoin/pharmacology
- Wnt Proteins/metabolism*
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 16938888 Full text @ Proc. Natl. Acad. Sci. USA
- CTD
- 16938888
Citation
Nadauld, L.D., Chidester, S., Shelton, D.N., Rai, K., Broadbent, T., Sandoval, I.T., Peterson, P.W., Manos, E.J., Ireland, C.M., Yost, H.J., and Jones, D.A. (2006) Dual roles for adenomatous polyposis coli in regulating retinoic acid biosynthesis and Wnt during ocular development. Proceedings of the National Academy of Sciences of the United States of America. 103(36):13409-13414.
Abstract
Congenital hypertrophy/hyperplasia of the retinal pigmented epithelium is an ocular lesion found in patients harboring mutations in the adenomatous polyposis coli (APC) tumor suppressor gene. We report that Apc-deficient zebrafish display developmental abnormalities of both the lens and retina. Injection of dominant-negative Lef reduced Wnt signaling in the lens but did not rescue retinal differentiation defects. In contrast, treatment of apc mutants with all-trans retinoic acid rescued retinal differentiation defects but had no apparent effect on the lens. We identified Rdh5 as a retina-specific retinol dehydrogenase controlled by APC. Morpholino knockdown of Rdh5 phenocopied the apc mutant retinal differentiation defects and was rescued by treatment with exogenous all-trans retinoic acid. Microarray analyses of apc mutants and Rdh5 morphants revealed a profound overlap in the transcriptional profile of these embryos. These findings support a model wherein Apc serves a dual role in regulating Wnt and retinoic acid signaling within the eye and suggest retinoic acid deficiency as an explanation for APC mutation-associated retinal defects such as congenital hypertrophy/hyperplasia of the retinal pigmented epithelium.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping