PUBLICATION
Characterization of the mRNA expression of StAR and steroidogenic enzymes in zebrafish ovarian follicles
- Authors
- Ings, J.S., and Van Der Kraak, G.J.
- ID
- ZDB-PUB-060526-4
- Date
- 2006
- Source
- Molecular reproduction and development 73(8): 943-954 (Journal)
- Registered Authors
- Keywords
- steroidogenesis, StAR, folliculogenesis, mRNA expression, ovary, zebrafish
- MeSH Terms
-
- Animals
- Aromatase/genetics
- Aromatase/metabolism
- Cholesterol Side-Chain Cleavage Enzyme/genetics
- Cholesterol Side-Chain Cleavage Enzyme/metabolism
- Chorionic Gonadotropin/metabolism
- Female
- Humans
- Hydroxysteroid Dehydrogenases/genetics
- Hydroxysteroid Dehydrogenases/metabolism
- Lyases/genetics
- Lyases/metabolism
- Ovarian Follicle/physiology*
- Phosphoproteins/genetics
- Phosphoproteins/metabolism*
- RNA, Messenger/metabolism*
- Zebrafish*/anatomy & histology
- Zebrafish*/physiology
- PubMed
- 16700073 Full text @ Mol. Reprod. Dev.
Citation
Ings, J.S., and Van Der Kraak, G.J. (2006) Characterization of the mRNA expression of StAR and steroidogenic enzymes in zebrafish ovarian follicles. Molecular reproduction and development. 73(8):943-954.
Abstract
The objective of this study was to investigate the levels of expression of steroid biosynthetic enzymes and steroidogenic acute regulatory protein (StAR) at different stages of ovarian follicular development in zebrafish (Danio rerio), and to investigate the sites within the steroid biosynthetic pathway that may be regulated by gonadotropins. Ovarian follicles of sexually mature fish were separated into primary, previtellogenic, vitellogenic, and mature stages and the expression of StAR, P450 side chain cleavage (P450scc), 3beta-hydroxysteroid dehydrogenase (3beta-HSD), P450 hydroxylase/lyase (P450c17), 17beta-hydroxysteroid dehydrogenase type 1 (17beta-HSD1), 17beta-hydroxysteroid dehydrogenase type 3 (17beta-HSD3), and P450 aromatase (P450aromA) was determined by Real time RT-PCR. The expression of all genes changed significantly as follicles grew, with a decrease in the expression of StAR, P450scc, 3beta-HSD and P450c17 with maturation, and an increase in the expression of 17beta-HSD3 during vitellogenesis and 17beta-HSD1 and P450aromA during previtellogenesis. In vitro incubation of vitellogenic follicles demonstrated that the expression of StAR, 17beta-HSD3, and P450aromA increased in response to hCG, and decreased in the absence of hCG. In contrast, the expression of P450scc, 3beta-HSD, P450c17, and 17beta-HSD1 remained constant between treatments and over time. Testosterone and estradiol production in the culture medium was stimulated by human chorionic gonadotropin (hCG). These experiments aid in the characterization of the roles and regulation of steroids throughout ovarian development, and suggest that gonadotropins play a key role in the regulation of StAR, 17beta-HSD3, and P450aromA in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping